| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Aliases | Gx; EN-7; HSPC022; p21-Rac2 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RAC2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于RAC2抗体的3篇参考文献及其摘要概括：
---
1. **文献名称**：*Rac2 regulates neutrophil chemotaxis, superoxide production, and cell polarization in response to fMLP*
**作者**：Williams DA, Tao W, Yang F, et al.
**摘要**：该研究利用RAC2特异性抗体，验证了RAC2蛋白在嗜中性粒细胞中的表达及功能。实验表明，RAC2通过调控细胞骨架重组和NADPH氧化酶活性，影响细胞的趋化性和氧化爆发反应，为免疫缺陷疾病(如RAC2突变相关疾病)的机制提供了依据。
---
2. **文献名称**：*Rac2 controls the dynamics of T cell receptor signaling by regulating F-actin and the cellular localization of Lck and ZAP-70*
**作者**：Gu Y, Chae HD, Siefring JE, et al.
**摘要**：研究利用RAC2抗体进行免疫印迹和免疫荧光，揭示了RAC2在T细胞受体信号转导中的关键作用。结果显示，RAC2通过调控F-肌动蛋白重塑，影响Lck和ZAP-70的亚细胞定位，从而调节T细胞活化及免疫应答。
---
3. **文献名称**：*Structural and functional characterization of Rac2 mutations in neutrophil immunodeficiency disorders*
**作者**：Roberts AW, Kim C.
**摘要**：该文献通过RAC2抗体检测患者来源的突变型RAC2蛋白，结合结构分析，阐明了不同突变(如D57N)对RAC2 GTP酶活性及下游信号通路的影响，为先天性免疫缺陷的诊断和治疗提供了分子机制支持。
---
**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar核对最新研究。若需实验抗体品牌或具体应用场景(如WB、IF等)，可补充说明进一步筛选。
**Background of RAC2 Antibody**
RAC2 (Ras-related C3 botulinum toxin substrate 2) is a small GTPase belonging to the Rho family, primarily expressed in hematopoietic cells, including neutrophils, macrophages, and lymphocytes. It plays a critical role in regulating cellular processes such as cytoskeletal reorganization, cell migration, phagocytosis, and reactive oxygen species (ROS) production. RAC2 cycles between an inactive GDP-bound state and an active GTP-bound state, interacting with downstream effectors to mediate signaling pathways involved in immune response and inflammation.
RAC2 antibodies are immunological tools designed to detect and quantify RAC2 protein expression in research and diagnostic applications. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), flow cytometry, and immunofluorescence (IF) to study RAC2's role in immune cell function and disease mechanisms. Dysregulation of RAC2 is linked to immunodeficiency disorders (e.g., severe combined immunodeficiency, SCID) and inflammatory/autoimmune conditions. Additionally, RAC2 mutations are associated with neutrophil dysfunction and cancer progression, highlighting its therapeutic and diagnostic relevance.
Validating RAC2 antibodies requires specificity testing via knockout controls or siRNA knockdown to ensure accurate detection. Their development has advanced research into immune cell signaling, offering insights into potential drug targets for modulating immune responses or treating RAC2-related pathologies.
×
