| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | BERP; HAC1; RNF22; RNF97 |
| WB Predicted band size | 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human TRIM3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及TRIM3抗体的代表性文献摘要(人工整理，非真实文献，供参考)：
1. **文献名称**：TRIM3 regulates neuronal excitability through ubiquitination of potassium channels
**作者**：Lee et al. (2018)
**摘要**：研究利用TRIM3特异性抗体，发现TRIM3通过泛素化修饰调控钾离子通道蛋白稳定性，影响神经元电活动，可能与癫痫病理相关。
2. **文献名称**：TRIM3 suppresses tumor metastasis by targeting β-catenin in hepatocellular carcinoma
**作者**：Zhang et al. (2020)
**摘要**：通过TRIM3抗体进行免疫共沉淀实验，揭示TRIM3通过促进β-catenin降解抑制肝癌细胞侵袭转移，其表达水平与患者预后正相关。
3. **文献名称**：TRIM3 modulates autophagosome-lysosome fusion via binding LC3
**作者**：Wang et al. (2021)
**摘要**：研究使用TRIM3抗体进行免疫荧光共定位，证明TRIM3通过结合自噬标志蛋白LC3调控自噬体-溶酶体融合过程，影响细胞稳态。
4. **文献名称**：Developmental expression of TRIM3 in mouse brain revealed by antibody-based staining
**作者**：Chen et al. (2019)
**摘要**：通过TRIM3抗体的免疫组化分析，发现TRIM3在小鼠胚胎大脑皮层神经元中高表达，提示其在神经发育中的潜在作用。
注：以上文献信息为示例性质，实际文献需通过PubMed或Web of Science检索确认。
The TRIM3 antibody targets Tripartite Motif-containing protein 3 (TRIM3), a member of the TRIM family characterized by conserved RING, B-box, and coiled-coil domains. TRIM3 plays diverse roles in cellular processes, including protein ubiquitination, intracellular signaling, and vesicular trafficking. It functions as an E3 ubiquitin ligase, regulating substrate degradation via the ubiquitin-proteasome system. TRIM3 is highly expressed in the brain, where it influences neuronal development, synaptic plasticity, and autophagy. Dysregulation of TRIM3 has been implicated in neurological disorders, such as Alzheimer’s disease, and cancers, including gliomas and hepatocellular carcinoma, where it may act as a tumor suppressor or promoter depending on context.
TRIM3 antibodies are essential tools for detecting TRIM3 expression and localization in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They aid in studying TRIM3's interaction partners, post-translational modifications, and mechanistic roles in disease pathways. Recent studies highlight TRIM3's involvement in regulating apoptosis, cell cycle progression, and immune responses, underscoring its therapeutic potential. Validated antibodies help clarify its dual roles in different cancers and neurological conditions, making it a focus for biomarker discovery and targeted therapy development.
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