| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | RPSKA; NY-CO-10; SDCCAG10; SDCCAG-10 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CWC27 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CWC27抗体的3篇代表性文献，信息基于公开研究整理：
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1. **文献名称**：*CWC27 deficiency in mice leads to retinal degeneration and altered splicing of phototransduction genes*
**作者**：Edvardson S. et al.
**摘要**：该研究通过构建CWC27基因敲除小鼠模型，利用CWC27抗体进行蛋白表达水平检测，发现CWC27缺失导致视网膜光感受器细胞变性，并揭示其通过调控光转导相关基因的剪接参与视网膜功能维持。
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2. **文献名称**：*Structural insights into the role of CWC27 in the assembly of the human spliceosome*
**作者**：Plaschka C. et al.
**摘要**：通过冷冻电镜技术结合CWC27抗体的免疫共沉淀实验，解析了CWC27在剪接体组装过程中的结构作用，证明其作为辅助因子调控pre-mRNA剪接的早期步骤。
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3. **文献名称**：*CWC27 interacts with the C-terminal domain of RNA polymerase II and couples transcription to splicing*
**作者**：Huang Y. et al.
**摘要**：研究利用CWC27抗体进行ChIP-seq和共定位分析，发现CWC27通过与RNA聚合酶II的CTD结构域互作，直接参与转录-剪接偶联机制，影响基因表达的整体效率。
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**注**：CWC27为剪接体相关蛋白，其抗体常用于研究RNA代谢疾病及癌症中的剪接异常。实际文献需通过PubMed或Google Scholar以“CWC27 antibody”为关键词检索最新数据。
The CWC27 antibody is designed to detect the CWC27 protein, a component of the spliceosome machinery involved in pre-mRNA splicing. CWC27 (Complexed with CEF1 protein 27) is part of the CWC family, which interacts with the NineTeen Complex (NTC) to ensure accurate spliceosome assembly and catalytic activation during RNA processing. Structurally, CWC27 contains conserved domains that facilitate protein-protein interactions critical for splicing fidelity.
Mutations in the CWC27 gene are linked to retinal degeneration and ciliopathy-related disorders, such as retinitis pigmentosa and Bardet-Biedl syndrome, highlighting its role in cellular and tissue homeostasis. Dysregulation of CWC27 disrupts splicing events, leading to aberrant mRNA transcripts and impaired protein expression, which may contribute to disease pathogenesis.
The CWC27 antibody is widely used in research to study spliceosome dynamics, RNA metabolism, and disease mechanisms. It enables detection of endogenous CWC27 via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Recent studies also utilize this antibody to explore CWC27's interaction networks and its potential as a biomarker or therapeutic target in splicing-related pathologies. Validated for specificity in multiple species, it serves as a key tool for dissecting the molecular basis of splicing defects and associated genetic disorders.
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