| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAGOH |
| Uniprot No | P61326 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-146aa |
| 氨基酸序列 | MESDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEGLRVFYYLVQDLKCLVFSLIGLHFKIKPI |
| 预测分子量 | 44.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAGOH Recombinant Protein**
MAGOH (Mago-Nashi homolog) is a conserved core component of the exon junction complex (EJC), a multiprotein assembly critical for post-transcriptional RNA processing. The EJC plays essential roles in mRNA splicing, export, translation, and surveillance by marking splice junctions and recruiting downstream effectors. MAGOH, along with its binding partners Y14 and eIF4AIII, stabilizes the EJC on spliced mRNAs, influencing processes such as nonsense-mediated decay (NMD) and localization. Dysregulation of MAGOH is linked to developmental defects, neurological disorders, and cancers, underscoring its biological significance.
Recombinant MAGOH protein is engineered using genetic cloning techniques, typically expressed in *E. coli* or mammalian systems to ensure proper folding and functionality. It is purified via affinity chromatography, often tagged with histidine or GST for ease of isolation. This recombinant tool enables detailed studies of EJC assembly, RNA-protein interactions, and molecular mechanisms underlying diseases. Researchers leverage MAGOH recombinant protein to investigate its role in stem cell differentiation, tumorigenesis, and neural development, as well as to screen therapeutic agents targeting EJC-related pathways. Its application extends to structural studies, antibody production, and functional assays, making it a vital resource in molecular and biomedical research.
Overall, MAGOH recombinant protein serves as a key reagent for unraveling the complexities of RNA biology and disease mechanisms tied to EJC dysfunction.
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