| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | CAPC; CAP-C; SMC-4; SMC4L1; hCAP-C |
| WB Predicted band size | 147 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SMC4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SMC4抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*"SMC4. a subunit of the condensin complex, is essential for proper chromosome segregation in mitosis and meiosis"*
**作者**：Hirano T, et al.
**摘要**：该研究利用SMC4特异性抗体，通过免疫荧光和Western blot技术，揭示了SMC4在染色体凝缩和有丝分裂中的关键作用。实验表明，SMC4缺失会导致染色体分离异常，强调了其在维持基因组稳定性中的重要性。
2. **文献名称**：*"Overexpression of SMC4 predicts poor prognosis in hepatocellular carcinoma and promotes cell proliferation"*
**作者**：Li Y, et al.
**摘要**：通过免疫组化(使用SMC4抗体)和临床数据分析，发现SMC4在肝细胞癌中显著高表达，且与患者不良预后相关。功能实验表明，SMC4通过调控细胞周期蛋白促进肿瘤细胞增殖。
3. **文献名称**：*"Condensin subunit SMC4 interacts with replication protein A and is required for DNA repair"*
**作者**：Wu N, et al.
**摘要**：本研究利用SMC4抗体进行免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)，证明SMC4与DNA修复蛋白RPA直接结合，参与DNA损伤修复过程，揭示了其在基因组维护中的新功能。
这些研究均通过SMC4抗体验证了该蛋白在染色体动态、癌症进展及DNA修复中的生物学功能。如需具体文献来源，可进一步检索PubMed或相关期刊数据库。
The SMC4 antibody targets the Structural Maintenance of Chromosomes 4 (SMC4) protein, a key component of the condensin complex essential for chromosome organization and stability during cell division. SMC4 belongs to the SMC protein family, which includes ATPases involved in DNA repair, recombination, and chromosome condensation. The condensin complex, comprising SMC4. SMC2. and non-SMC subunits, ensures proper chromatin compaction and sister chromatid resolution during mitosis and meiosis. Dysregulation of SMC4 is linked to genomic instability, a hallmark of cancer and developmental disorders.
Antibodies against SMC4 are widely used in research to study chromosome dynamics, cell cycle regulation, and mitotic errors. They enable detection of SMC4 expression levels, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Studies utilizing SMC4 antibodies have revealed its overexpression in certain cancers, correlating with poor prognosis, and its role in maintaining mitotic fidelity. These tools also aid in exploring condensin's involvement in 3D genome organization and transcriptional regulation. Validated SMC4 antibodies are critical for elucidating mechanisms underlying chromosomal abnormalities and potential therapeutic targets.
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