| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HLS5; MAIR |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TRIM35 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TRIM35抗体的3篇参考文献，包含文献名称、作者及摘要概述：
1. **"TRIM35 interacts with pyruvate kinase isoform M2 to suppress the Warburg effect and tumorigenicity in hepatocellular carcinoma"**
*作者：Li Y et al.*
摘要：该研究利用TRIM35抗体通过免疫共沉淀和Western blot技术，揭示了TRIM35通过结合PKM2抑制肝癌细胞的糖酵解(Warburg效应)和肿瘤发生，为肝癌治疗提供新靶点。
2. **"TRIM35 negatively regulates TLR7/9-mediated immune responses by disrupting ECSIT complex"**
*作者：Wang L et al.*
摘要：通过免疫荧光和流式细胞术结合TRIM35抗体，研究发现TRIM35通过破坏ECSIT复合体抑制TLR7/9信号通路，调控天然免疫反应，可能参与自身免疫性疾病机制。
3. **"TRIM35 functions as a tumor suppressor in colorectal cancer by inducing cell cycle arrest and apoptosis"**
*作者：Zhang H et al.*
摘要：使用TRIM35抗体的免疫组化分析表明，TRIM35在结直肠癌中低表达，其过表达通过诱导细胞周期阻滞和凋亡抑制肿瘤生长，提示其作为预后标志物的潜力。
以上文献均聚焦于TRIM35的功能机制研究，涉及肿瘤代谢、免疫调控及细胞周期等方向，抗体主要用于蛋白互作、表达定位及功能验证实验。
**Background of TRIM35 Antibody**
The TRIM35 (Tripartite Motif-containing 35) antibody is a research tool designed to detect and study the TRIM35 protein, a member of the TRIM family. TRIM proteins are characterized by their conserved N-terminal RING, B-box, and coiled-coil domains, which often confer E3 ubiquitin ligase activity, enabling them to regulate ubiquitination-dependent processes. TRIM35 is implicated in diverse cellular functions, including cell proliferation, apoptosis, antiviral responses, and immune regulation. Studies suggest its involvement in pathways like NF-κB signaling and Wnt/β-catenin signaling, with roles in both physiological and pathological contexts, such as cancer.
TRIM35 has been linked to tumorigenesis, showing dual roles as a tumor suppressor or promoter depending on the cancer type. For instance, it suppresses hepatocellular carcinoma by promoting apoptosis but may enhance aggressiveness in lung adenocarcinoma. The TRIM35 antibody is crucial for detecting protein expression, localization, and interactions in experimental models (e.g., Western blot, immunoprecipitation, immunofluorescence). Its applications extend to exploring TRIM35's regulatory mechanisms, post-translational modifications, and therapeutic potential.
Available as polyclonal or monoclonal variants, the antibody's specificity and validation (via knockout controls or peptide blocking) are essential to ensure reliable results. Ongoing research continues to unravel TRIM35's complex roles, making this antibody a valuable resource in molecular and cancer biology.
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