| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III subunit C4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47 kDa subunit, RPC53 homolog, POLR3D, BN51, BN51T |
| Entrez GeneID | 661 |
| WB Predicted band size | 44.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POLR3D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the Central region of human POLR3D. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于VMP1抗体的3篇参考文献的简要信息(基于公开研究整理，非真实文献，仅供参考格式)：
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1. **文献名称**: *VMP1 modulates autophagy and pancreatic cancer progression through mTOR signaling*
**作者**: Zhang L, et al.
**摘要**: 研究利用VMP1特异性抗体揭示VMP1在胰腺癌细胞自噬调控中的作用，发现其通过抑制mTOR通路促进肿瘤细胞存活，抗体用于Western blot和免疫组化验证蛋白表达。
2. **文献名称**: *VMP1 antibody-based detection reveals its essential role in autophagosome-lysosome fusion*
**作者**: Tanaka K, et al.
**摘要**: 通过VMP1抗体进行免疫荧光和共定位分析，证实VMP1是自噬体-溶酶体膜融合的关键因子，敲除VMP1导致自噬流阻断，抗体特异性经siRNA验证。
3. **文献名称**: *VMP1 as a novel biomarker for neurodegenerative diseases: Insights from antibody validation*
**作者**: Rodriguez-Blanco J, et al.
**摘要**: 研究开发并验证了一种高特异性VMP1多克隆抗体，发现阿尔茨海默病模型中VMP1表达异常升高，抗体应用于ELISA和脑组织免疫染色，提示其病理关联性。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“VMP1 antibody”或“VMP1 autophagy”，并筛选近年研究。部分经典文献可能涉及抗体应用细节(如公司货号、实验条件等)。
VMP1 (Vacuole Membrane Protein 1) is a conserved transmembrane protein initially identified for its role in regulating the formation of autophagosomes, critical structures in cellular autophagy. It localizes to endoplasmic reticulum (ER)-associated membranes and dynamically interacts with other autophagy-related proteins, such as ATG16L1 and WIPI2. to facilitate membrane expansion during autophagosome biogenesis. Beyond autophagy, VMP1 is implicated in maintaining ER morphology, lipid droplet dynamics, and membrane contact sites between the ER and Golgi apparatus. Dysregulation of VMP1 has been linked to diseases including cancer, neurodegenerative disorders, and pancreatitis, where impaired autophagy or ER stress contributes to pathogenesis.
VMP1 antibodies are essential tools for studying these processes. They enable the detection and localization of VMP1 in cells and tissues via techniques like immunofluorescence, Western blotting, and immunoprecipitation. Polyclonal and monoclonal VMP1 antibodies are generated using immunogenic peptide sequences or recombinant protein fragments, often validated for specificity using knockout cell lines. Recent studies employ these antibodies to explore VMP1's involvement in disease mechanisms, drug response, and its potential as a therapeutic target. However, challenges remain in standardizing antibody performance across experimental models, emphasizing the need for rigorous validation in diverse biological contexts.
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