| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MBD3 |
| Uniprot No | O95983 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-291aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMERKRWE CPALPQGWER EEVPRRSGLS AGHRDVFYYS PSGKKFRSKP QLARYLGGSM DLSTFDFRTG KMLMSKMNKS RQRVRYDSSN QVKGKPDLNT ALPVRQTASI FKQPVTKITN HPSNKVKSDP QKAVDQPRQL FWEKKLSGLN AFDIAEELVK TMDLPKGLQG VGPGCTDETL LSAIASALHT STMPITGQLS AAVEKNPGVW LNTTQPLCKA FMVTDEDIRK QEELVQQVRK RLEEALMADM LAHVEELARD GEAPLDKACA EDDDEEDEEE EEEEPDPDPE MEHV |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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MBD3 (methyl-CpG-binding domain protein 3) is a member of the methyl-CpG-binding protein family, which plays a critical role in epigenetic regulation by interpreting DNA methylation signals. It is a key component of the NuRD (nucleosome remodeling and deacetylase) complex, linking DNA methylation to chromatin remodeling and transcriptional repression. Unlike other MBD family members (e.g., MBD2 or MeCP2), MBD3 lacks strong methyl-CpG binding activity due to amino acid substitutions in its methyl-binding domain, suggesting a distinct regulatory mechanism. Instead, it facilitates NuRD complex assembly and mediates interactions with chromatin modifiers like histone deacetylases (HDACs) and ATP-dependent remodelers.
Recombinant MBD3 protein is engineered for in vitro studies to dissect its structural and functional roles. Produced via bacterial or mammalian expression systems, it retains domains critical for NuRD interaction, including the conserved MBD and coiled-coil regions. Researchers utilize recombinant MBD3 to investigate its role in gene silencing, cell differentiation, and embryonic development. For example, studies show MBD3-NuRD dynamically regulates pluripotency in stem cells by balancing self-renewal and differentiation signals. It also participates in tumor suppression and oncogenic pathways, making it relevant to cancer research.
Purified recombinant MBD3 is often tagged (e.g., His, GST) for affinity-based assays, co-immunoprecipitation, or chromatin-binding studies. Its application extends to drug discovery, particularly in targeting epigenetic dysregulation. By studying MBD3 interactions, scientists aim to uncover mechanisms underlying diseases like cancer, neurodevelopmental disorders, and metabolic syndromes, highlighting its importance in both basic and translational research.
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