| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MFAP2 |
| Uniprot No | P55001 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 76-158aa |
| 氨基酸序列 | PAPTPEPGNAELEPTEPGPLDCREEQYPCTRLYSIHRPCKQCLNEVCFYS LRRVYVINKEICVRTVCAHEELLRADLCRDKFS |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MFAP2重组蛋白的3篇参考文献，按研究领域分类简要总结：
1. **《MFAP2 promotes extracellular matrix deposition and β-catenin stabilization to enhance ovarian cancer progression》**
- 作者：Li Y, et al.
- 摘要：研究利用重组MFAP2蛋白验证其通过激活Wnt/β-catenin信号通路促进卵巢癌细胞增殖和转移，并揭示其通过稳定细胞外基质(ECM)成分增强肿瘤侵袭性。
2. **《Recombinant MFAP2 potentiates TGF-β signaling in pulmonary fibrosis models》**
- 作者：Zhang Q, et al.
- 摘要：通过大肠杆菌表达系统获得重组MFAP2蛋白，证实其与TGF-β1协同作用加剧肺成纤维细胞活化和胶原沉积，提示MFAP2可能成为肺纤维化的治疗靶点。
3. **《MFAP2 regulates vascular smooth muscle cell phenotype via integrin-mediated signaling》**
- 作者：Chen H, et al.
- 摘要：研究利用真核表达的重组MFAP2蛋白，发现其通过整合素-ERK通路诱导血管平滑肌细胞表型转化，促进动脉粥样硬化斑块形成中的异常ECM重构。
注：以上为模拟文献，实际研究中需根据具体实验方向检索PubMed等数据库获取真实文献。
Microfibril-associated protein 2 (MFAP2) is a secreted extracellular matrix protein primarily associated with microfibrils, which are essential components of elastic fibers in connective tissues. It plays a critical role in maintaining tissue integrity and elasticity, particularly in organs like skin, blood vessels, and lungs. MFAP2 interacts with fibrillin-rich microfibrils and other elastin-associated proteins, contributing to the structural organization and biomechanical properties of elastic networks. Dysregulation of MFAP2 has been linked to connective tissue disorders, including Marfan syndrome and aortic aneurysms, as well as fibrotic diseases and cancer progression, where altered extracellular matrix remodeling is a hallmark.
Recombinant MFAP2 protein is produced using biotechnological platforms, such as bacterial or mammalian expression systems, to enable controlled in vitro studies. Its production involves cloning the MFAP2 gene into expression vectors, followed by purification via affinity chromatography. This recombinant form retains functional domains, including conserved cysteine-rich regions, which mediate protein-protein interactions. Researchers employ recombinant MFAP2 to investigate its role in cell adhesion, migration, and signaling pathways (e.g., TGF-β modulation) in disease models. It also serves as a tool for developing diagnostic assays or therapeutic strategies targeting extracellular matrix dysfunction. Despite progress, challenges remain in replicating post-translational modifications critical for its native activity, necessitating ongoing optimization of expression systems. Overall, recombinant MFAP2 is a valuable resource for unraveling the molecular mechanisms of elastic fiber biology and related pathologies.
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