纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MFAP3 |
Uniprot No | P55082 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 19-147aa |
氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSAFVLEDV DFDQMVSLEA NRSSYNASFP SSFELSASSH SDDDVIIAKE GTSVSIECLL TASHYEDVHW HNSKGQQLDG RSRGGKWLVS DNFLNITNVA FDDRGLYTCF VTSPIRASYS VTLRVIFTSG DM |
预测分子量 | 17 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MFAP3重组蛋白的3篇参考文献,包含文献名称、作者及摘要概括内容:
1. **"Recombinant human MFAP3 promotes fibroblast adhesion and migration via integrin signaling"**
- **作者**: Zhang Y, et al.
- **摘要**: 研究报道了重组人MFAP3蛋白的制备及其在成纤维细胞中的作用。通过体外实验发现,MFAP3通过整合素信号通路增强细胞黏附和迁移,提示其在组织修复和纤维化中的潜在功能。
2. **"Expression and purification of MFAP3 in a prokaryotic system for functional characterization"**
- **作者**: Lee S, Kim JH.
- **摘要**: 描述了一种在原核系统中高效表达和纯化重组MFAP3蛋白的方法,并验证其结构稳定性。研究为MFAP3的体外功能分析(如配体结合和酶活性)提供了技术基础。
3. **"MFAP3 interacts with extracellular matrix components and regulates TGF-β signaling in cancer progression"**
- **作者**: Wang L, et al.
- **摘要**: 研究发现重组MFAP3蛋白通过与胶原蛋白和纤连蛋白相互作用,调控TGF-β信号通路,促进肿瘤细胞侵袭和转移,揭示了其在癌症微环境中的潜在作用。
(注:上述文献为模拟示例,实际引用需根据具体文献调整。)
MFAP3 (Microfibril-associated protein 3) is a secreted glycoprotein that plays a role in extracellular matrix (ECM) organization and cellular interactions. It is part of the microfibrillar network, which provides structural support to tissues and regulates cell signaling. MFAP3 is primarily associated with elastic fibers and microfibrils, interacting with proteins like fibrillin-1. elastin, and integrins. These interactions contribute to ECM stability, tissue elasticity, and mechanotransduction processes. Dysregulation of MFAP3 has been implicated in connective tissue disorders, cardiovascular diseases, and certain cancers, though its precise molecular mechanisms remain under investigation.
Recombinant MFAP3 protein is produced using biotechnological platforms, such as bacterial or mammalian expression systems, to generate purified, bioactive forms of the protein for research and therapeutic applications. Its production enables studies on MFAP3's structural properties, binding partners, and functional roles in development and disease. For example, recombinant MFAP3 has been utilized to investigate its involvement in TGF-β signaling modulation, cell adhesion, and tissue remodeling. It also serves as a potential biomarker or therapeutic target in fibrosis and tumor microenvironment studies.
Current research focuses on elucidating MFAP3's dual roles in ECM homeostasis and pathological processes. Its recombinant form provides a tool to explore tissue-specific interactions, screen drug candidates, and develop engineered biomaterials for regenerative medicine. Challenges include optimizing post-translational modifications to mimic native protein behavior and clarifying its context-dependent functions across different disease models. Ongoing studies aim to bridge gaps in understanding how MFAP3 balances structural integrity with dynamic cellular communication in health and disease.
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