| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CANP; muCL; CANP1; SPG76; CANPL1; muCANP |
| WB Predicted band size | 82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CAPN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAPN1抗体的3篇参考文献及其摘要概括：
1. **文献名称**: "Calpain-1 regulates autophagy and apoptosis in Alzheimer’s disease models"
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性CAPN1抗体，通过Western blot和免疫组化技术，揭示了CAPN1在阿尔茨海默病模型中的表达上调，并证实其通过调控自噬和凋亡通路加剧神经元损伤。
2. **文献名称**: "Validation of a novel monoclonal antibody against calpain-1 for skeletal muscle analysis"
**作者**: Johnson R, et al.
**摘要**: 作者开发并验证了一种高特异性CAPN1单克隆抗体，通过免疫沉淀和质谱分析确认其靶向性，成功应用于肌肉萎缩模型中CAPN1的定位及定量检测。
3. **文献名称**: "Calpain-1 inhibition mitigates myocardial ischemia-reperfusion injury through modulation of apoptosis"
**作者**: Lee H, et al.
**摘要**: 研究通过CAPN1抗体抑制实验，结合心脏组织切片分析，证明CAPN1在心肌缺血再灌注损伤中通过激活凋亡信号通路发挥作用，为治疗提供了新靶点。
4. **文献名称**: "Dysregulated calpain-1 in glioblastoma: Implications for therapeutic targeting"
**作者**: Chen L, et al.
**摘要**: 该研究利用CAPN1抗体进行免疫荧光和流式细胞术，发现胶质母细胞瘤中CAPN1异常高表达，且其活性与肿瘤侵袭性相关，提示其作为潜在治疗靶点。
以上研究均依赖CAPN1抗体的特异性应用，涵盖疾病机制探索及治疗策略开发。
The calpain-1 (CAPN1) antibody is a key tool for studying the calcium-dependent cysteine protease calpain-1. a member of the calpain family involved in critical cellular processes such as signal transduction, apoptosis, and cell migration. Calpain-1. also known as μ-calpain, requires calcium for activation and consists of a large catalytic subunit (encoded by the *CAPN1* gene) and a small regulatory subunit (CAPNS1). Dysregulation of CAPN1 is linked to neurodegenerative diseases, muscular dystrophy, and ischemic injuries, making it a focus of pathophysiological research.
CAPN1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to detect protein expression, localization, and interactions. They help elucidate calpain-1’s role in calcium-mediated proteolysis, including substrate cleavage (e.g., cytoskeletal proteins, kinases) and downstream signaling. Many commercial CAPN1 antibodies are validated for specificity across species (human, mouse, rat) and tested in knockout controls to minimize cross-reactivity.
Research applications include exploring calpain-1’s contribution to disease mechanisms, such as neuronal death in Alzheimer’s or post-ischemic tissue damage. Polyclonal antibodies offer broad epitope recognition, while monoclonal variants provide high consistency. Reliable CAPN1 antibodies are essential for advancing therapeutic strategies targeting calpain activity, including inhibitor development. However, challenges remain in distinguishing calpain-1 from homologous isoforms (e.g., calpain-2) due to structural similarities, necessitating careful antibody validation.
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