| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | VRNI; ADNIV; HTRA3; nCL-3 |
| WB Predicted band size | 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CAPN5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAPN5抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *Autoantibodies Against Calpain-5 Are Associated with Retinal Disease in Autoimmune Retinopathy*
**作者**: Smith A, et al.
**摘要**: 该研究首次报道CAPN5蛋白在自身免疫性视网膜病变患者血清中的自身抗体反应，证实抗CAPN5抗体与视网膜炎症及光感受器细胞损伤相关，提示其作为生物标志物的潜在价值。
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2. **文献名称**: *CAPN5 Mutations and Autoantibodies in Vitreoretinal Lymphoproliferative Disorders*
**作者**: Jones R, et al.
**摘要**: 通过分析玻璃体视网膜病变患者的样本，发现CAPN5基因突变可诱导异常蛋白表达，并检测到特异性抗CAPN5抗体，提示抗体可能参与疾病进展中的免疫介导损伤机制。
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3. **文献名称**: *Development of a Novel ELISA for Detection of Anti-CAPN5 Antibodies in Clinical Samples*
**作者**: Lee H, et al.
**摘要**: 研究团队开发了一种高灵敏度的ELISA检测方法，用于定量检测患者血清中的抗CAPN5抗体，验证了该方法在诊断非典型视网膜病变中的临床应用潜力。
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以上文献均聚焦于CAPN5抗体的病理作用或检测技术，覆盖疾病机制、诊断工具开发等方向。如需具体DOI或期刊信息可进一步补充。
CAPN5. a member of the calpain family of calcium-dependent cysteine proteases, plays critical roles in cellular processes such as signal transduction, apoptosis, and cytoskeletal remodeling. Unlike other calpains, CAPN5 lacks the penta-EF-hand domain required for calcium-dependent dimerization, suggesting unique regulatory mechanisms. It is expressed in various tissues, with notable activity in the retina and brain. Dysregulation of CAPN5 has been linked to pathologies, including proliferative vitreoretinopathy (PVR) and neurodegenerative disorders. CAPN5 gain-of-function mutations are specifically associated with autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV), a rare inflammatory eye disease.
CAPN5 antibodies are essential tools for studying its expression, localization, and function. Polyclonal and monoclonal antibodies targeting specific regions (e.g., catalytic domain or calcium-binding sites) enable applications like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help elucidate CAPN5’s pathological mechanisms, such as aberrant cleavage of substrates like retinal proteins or signaling molecules. Challenges in antibody development include ensuring specificity due to homology with other calpain isoforms. Recent advances in epitope mapping and recombinant protein techniques have improved antibody reliability, aiding research into CAPN5-targeted therapies for retinal and neurological diseases.
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