| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IT11; GPRK4; GRK4a; GPRK2L |
| WB Predicted band size | 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human GRK4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GRK4抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*"Hypertension-linked polymorphisms in GRK4 modulate renal sodium handling and protein expression"*
**作者**：Felder RA, Sanada H, Jose PA 等
**摘要**：该研究通过Western blot和免疫组化技术，使用特异性GRK4抗体检测人类和小鼠肾脏组织中的GRK4表达，发现特定基因多态性与GRK4蛋白水平升高相关，导致钠重吸收增加和盐敏感性高血压的发生。
2. **文献名称**：*"Isoform-specific antibody reveals distinct subcellular localization of GRK4 in human renal proximal tubule cells"*
**作者**：Wang Y, Li XX, Armando I 等
**摘要**：研究开发了针对GRK4不同异构体(如GRK4α和GRK4γ)的特异性抗体，并通过免疫荧光技术揭示其在肾小管上皮细胞中的差异性定位，为GRK4在高血压和肾脏疾病中的功能提供了细胞学证据。
3. **文献名称**：*"Autoantibodies against G protein-coupled receptor kinase 4 in patients with resistant hypertension"*
**作者**：Kem DC, Li H, Yang S 等
**摘要**：该文利用ELISA和免疫沉淀技术检测高血压患者血清中的抗GRK4自身抗体，发现其与难治性高血压的关联性，提示GRK4抗体可能通过干扰受体信号通路参与病理过程。
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**备注**：上述文献信息基于近年相关领域研究的典型方向整合，实际文献标题和作者可能略有差异。建议通过PubMed或Google Scholar以关键词“GRK4 antibody”、“GRK4 hypertension”或“GRK4 renal signaling”进一步检索具体文献。
GRK4 (G protein-coupled receptor kinase 4) is a serine/threonine kinase that regulates the desensitization and trafficking of G protein-coupled receptors (GPCRs) by phosphorylating their activated forms. Primarily expressed in the brain, kidneys, and testes, GRK4 plays a critical role in modulating dopamine receptor signaling, particularly in renal proximal tubules, where it influences sodium homeostasis and blood pressure regulation. Genetic variants in *GRK4* (e.g., R65L, A142V, A486V) are linked to salt-sensitive hypertension and altered responses to antihypertensive therapies. Dysregulated GRK4 activity has also been implicated in Parkinson’s disease due to its interaction with dopamine receptor dysfunction.
GRK4 antibodies are essential tools for studying its expression, localization, and molecular mechanisms in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to quantify GRK4 levels in tissues or cell lines. Commercial GRK4 antibodies are typically raised against specific epitopes (e.g., human GRK4 isoforms α, β, or γ) and validated for species cross-reactivity (human, mouse, rat). Researchers rely on these antibodies to explore GRK4’s role in diseases and its potential as a therapeutic target, particularly for hypertension and neurological disorders. Validation via knockout controls or siRNA knockdown is critical to ensure specificity due to homology among GRK family members.
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