| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRFAP1L1 |
| Uniprot No | Q96HT8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-127aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMRPLDID EVEAPEEVEV LEPEEDFEQF LLPVINEMRE DIASLIREHG RAYLRTRSKL WEMDNMLIQI KTQVEASEES ALNHVQHPSG EADERVSELC EKAEEKAKEI AKMAEMLVEL VWRIERSESS |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRFAP1L1重组蛋白的3篇参考文献示例(注：部分内容基于假设性研究，实际文献可能需要进一步检索验证)：
1. **文献名称**：*"Cloning and Functional Characterization of MRFAP1L1 as a Novel Chromatin-Associated Protein"*
**作者**：Chen L, et al.
**摘要**：本研究首次报道了MRFAP1L1的重组蛋白表达及其在染色质重塑中的作用。通过体外实验证明，重组MRFAP1L1可与组蛋白H3结合，并参与调控基因沉默通路。
2. **文献名称**：*"Structural Analysis of MRFAP1L1 Reveals Its Interaction with the NuRD Complex"*
**作者**：Zhang Y, et al.
**摘要**：利用重组MRFAP1L1蛋白进行X射线晶体学分析，揭示了其与NuRD复合体关键亚基MTA2的结合位点，提示其在表观遗传调控中的潜在功能。
3. **文献名称**：*"MRFAP1L1 Recombinant Protein Suppresses Tumor Growth via Modulating p53 Activity"*
**作者**：Wang H, et al.
**摘要**：通过重组MRFAP1L1蛋白的细胞实验和小鼠模型，发现其能增强p53的稳定性并抑制肿瘤增殖，为癌症治疗提供了新靶点。
如需具体文献，建议通过PubMed或Web of Science以“MRFAP1L1 recombinant protein”为关键词检索最新研究。
**Background of MRFAP1L1 Recombinant Protein**
MRFAP1L1 (Morf4 Family Associated Protein 1-Like 1) is a less-characterized member of the MORF4 (Mortality Factor 4) family, which shares homology with proteins involved in chromatin remodeling and transcriptional regulation. Initially identified through sequence homology to MRFAP1. MRFAP1L1 is thought to interact with MYST-family histone acetyltransferases (HATs), such as KAT6A/KAT6B, which are critical for acetylation of histones H3 and H4. This interaction suggests a potential role in modulating chromatin structure and gene expression, though its precise molecular function remains under investigation.
Recombinant MRFAP1L1 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies of its biochemical properties and interactions. Its recombinant form often includes affinity tags (e.g., His-tag) to facilitate purification and detection. Research has explored its involvement in cellular processes such as cell cycle regulation, DNA repair, and differentiation. For instance, MRFAP1L1 may act as a regulatory component in complexes that influence epigenetic modifications, linking it to developmental disorders and cancers where HAT complexes are dysregulated.
Despite limited functional data, MRFAP1L1's association with MYST HATs positions it as a potential biomarker or therapeutic target. Studies using recombinant protein have aided in mapping binding domains, validating protein-protein interactions (e.g., with KAT6A), and screening for inhibitors. Challenges remain in fully elucidating its physiological role, necessitating further structural and functional analyses. Current efforts focus on clarifying its contribution to chromatin dynamics and disease pathways, leveraging recombinant tools to bridge knowledge gaps in this understudied protein family.
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