| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2 |
| WB Predicted band size | 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAPK8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于MAPK8(JNK1)抗体的参考文献示例，涵盖其在不同研究中的应用：
1. **"Role of JNK1 in apoptosis through MAPK signaling"**
*Authors: Davis RJ, et al.*
摘要：该研究利用MAPK8特异性抗体，通过Western blot分析JNK1在应激诱导的细胞凋亡中的激活状态，证实其在磷酸化c-Jun和调控凋亡通路中的关键作用。
2. **"Characterization of JNK isoforms in neuronal differentiation"**
*Authors: Tournier C, et al.*
摘要：通过免疫荧光和免疫沉淀技术，使用MAPK8抗体比较JNK1与JNK2在神经元分化中的亚细胞定位差异，揭示JNK1特异性参与轴突导向信号传导。
3. **"Validation of a monoclonal MAPK8 antibody for flow cytometry"**
*Authors: Gupta S, et al.*
摘要：研究开发并验证了一种高特异性抗MAPK8单克隆抗体，证明其在流式细胞术中检测T淋巴细胞内JNK1表达水平的可靠性，适用于免疫细胞功能研究。
(注：以上为示例文献，实际引用需以具体论文数据为准。)
The mitogen-activated protein kinase 8 (MAPK8), also known as JNK1 (c-Jun N-terminal kinase 1), is a serine/threonine kinase involved in regulating cellular responses to stress, proliferation, differentiation, and apoptosis. It belongs to the JNK subfamily of MAPKs and is activated by upstream kinases (MAP2Ks) in response to cytokines, growth factors, or environmental stressors. Upon activation, MAPK8 phosphorylates downstream targets, including transcription factors like c-Jun, ATF2. and p53. influencing gene expression linked to inflammation, survival, or cell death. Dysregulation of MAPK8 signaling is implicated in cancer, neurodegenerative disorders, and inflammatory diseases.
MAPK8 antibodies are essential tools for detecting and quantifying MAPK8 protein levels, phosphorylation status, and subcellular localization in research. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specific antibodies targeting distinct epitopes (e.g., total MAPK8 vs. phosphorylated forms) enable studies of pathway activation under experimental conditions. These antibodies are critical for investigating MAPK8's role in disease mechanisms, drug discovery, and validating genetic or pharmacological interventions.
Commercial MAPK8 antibodies are typically raised in rabbits or mice, validated for specificity using knockout cell lines or siRNA-mediated silencing. Researchers must select antibodies based on species reactivity (human, mouse, rat), application compatibility, and post-translational modification targets (e.g., Thr183/Tyr185 phosphorylation). Proper controls, such as isotype-matched IgG and positive/negative cell lysates, are essential to ensure reliable results.
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