| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CMT2; RP1-261G23.6 |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MAD2L1BP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAD2L1BP抗体的3篇代表性文献(虚构示例，仅供参考格式)：
1. **"MAD2L1BP overexpression promotes hepatocellular carcinoma progression by impairing mitotic checkpoint"**
*作者：Wang X, et al.*
摘要：研究通过MAD2L1BP抗体检测肝癌组织中蛋白表达水平，发现其高表达与染色体不稳定及患者预后不良相关，提示其作为肝癌治疗潜在靶点。
2. **"Characterization of MAD2L1BP as a regulator of the spindle assembly checkpoint"**
*作者：Chen L, et al.*
摘要：利用MAD2L1BP特异性抗体进行免疫共沉淀实验，揭示其通过与MAD2蛋白相互作用调控有丝分裂检查点，影响细胞周期停滞及基因组稳定性。
3. **"MAD2L1BP antibody-based detection in gastric cancer diagnosis"**
*作者：Li Y, et al.*
摘要：开发高灵敏度MAD2L1BP抗体用于胃癌组织免疫组化分析，证实其表达水平与肿瘤转移和化疗耐药性显著相关，可作为诊断标志物。
(注：以上文献信息为模拟示例，实际引用需根据具体研究查找真实文献。)
The MAD2L1BP (MAD2L1 Binding Protein) antibody is a tool used to study the MAD2L1BP protein, also known as p31comet, which plays a critical role in regulating the spindle assembly checkpoint (SAC) during mitosis. MAD2L1BP interacts directly with MAD2 (Mitotic Arrest Deficient 2), a key component of the SAC that ensures proper chromosome segregation by delaying anaphase until all chromosomes are correctly attached to spindle microtubules. By binding to MAD2. MAD2L1BP facilitates the inactivation of the SAC, promoting the transition from metaphase to anaphase and ensuring timely mitotic exit. This dynamic interaction is essential for maintaining genomic stability and preventing aneuploidy, a hallmark of many cancers.
Research using MAD2L1BP antibodies has elucidated its dual role in both checkpoint silencing and cancer progression. Overexpression or dysregulation of MAD2L1BP is linked to checkpoint override, chromosomal instability, and tumorigenesis. The antibody is widely employed in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression levels, subcellular localization, and interactions in various cellular and tissue contexts. It has become indispensable for studying cell cycle regulation, mitotic errors, and mechanisms underlying cancer development. Additionally, its utility extends to drug discovery, where it helps evaluate compounds targeting the SAC for cancer therapy. Understanding MAD2L1BP's function through antibody-based assays continues to provide insights into mitotic control pathways and potential therapeutic interventions.
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