| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MLK1; MEKK9; PRKE1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MAP3K9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与MAP3K9(MEKK9)抗体相关的文献摘要概览：
1. **文献名称**：*MAP3K9 promotes metabolic reprogramming in hepatocellular carcinoma via enhancing Warburg effect*
**作者**：Li X et al.
**摘要**：该研究使用MAP3K9特异性抗体进行Western blot和免疫组化，发现MAP3K9通过激活HIF-1α/PDK1通路促进肝癌细胞糖酵解(Warburg效应)，从而驱动肿瘤生长和耐药性。
2. **文献名称**：*MEKK9 regulates ERK5 activation and promotes melanoma metastasis*
**作者**：Zhang Y et al.
**摘要**：通过shRNA敲低和MAP3K9抗体验证，研究揭示MEKK9通过磷酸化ERK5调控黑色素瘤细胞侵袭转移，其表达水平与患者预后不良显著相关。
3. **文献名称**：*A novel role of MAP3K9 in adipocyte differentiation and insulin resistance*
**作者**：Wang R et al.
**摘要**：利用MAP3K9抗体进行组织染色及Co-IP实验，发现MAP3K9通过调控PPARγ活性影响脂肪细胞分化，其异常表达与肥胖相关胰岛素抵抗密切相关。
注：以上为示例性文献，实际文献需通过PubMed/Google Scholar检索关键词“MAP3K9 antibody”或“MEKK9 function”获取。建议优先选择3年内发表的文献，并关注抗体应用场景(如WB/IHC/IF等实验方法)。
The MAP3K9 antibody is a crucial tool for studying the mitogen-activated protein kinase kinase kinase 9 (MAP3K9), a serine/threonine kinase involved in intracellular signaling pathways. MAP3K9. also known as MLK1 or mixed-lineage kinase 1. regulates stress-activated MAPK cascades, particularly the c-Jun N-terminal kinase (JNK) pathway. It plays roles in cellular processes like apoptosis, inflammation, and differentiation, with implications in cancer, neurodegenerative diseases, and metabolic disorders. Dysregulation of MAP3K9 has been linked to tumor progression, insulin resistance, and neuronal death.
Antibodies targeting MAP3K9 enable researchers to detect its expression, phosphorylation status, and subcellular localization in tissues or cultured cells using techniques such as Western blotting, immunohistochemistry, and immunofluorescence. These tools help elucidate MAP3K9's interaction networks, activation mechanisms, and downstream effectors. Commercial MAP3K9 antibodies are typically validated for specificity across human, mouse, and rat models, with monoclonal and polyclonal variants available.
Current research focuses on MAP3K9's dual roles as both a pro-survival and pro-apoptotic factor, depending on cellular context and stimuli. Its potential as a therapeutic target, particularly in cancers with MAP3K9 overexpression or mutations, drives antibody development for preclinical studies. However, challenges remain in distinguishing MAP3K9 isoforms and minimizing cross-reactivity with related kinases. Validated antibodies remain essential for advancing our understanding of MAP3K9's pathophysiological functions and therapeutic applications.
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