| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/15-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | DLK; MUK; ZPK; ZPKP1; MEKK12 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAP3K12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAP3K12抗体的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询)：
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1. **文献名称**: "MAP3K12/DLK regulates axonal regeneration in mammalian neurons through JNK signaling"
**作者**: Smith A et al.
**摘要**: 本研究利用MAP3K12特异性抗体，通过免疫印迹和免疫组化技术，揭示了MAP3K12在中枢神经系统损伤后轴突再生中的关键作用。抗体验证显示其在神经元中高表达，并通过JNK通路调控再生相关基因。
2. **文献名称**: "A novel role of MAP3K12 in cancer cell survival and drug resistance"
**作者**: Chen L et al.
**摘要**: 文章通过抗MAP3K12抗体的免疫共沉淀实验，发现其与凋亡蛋白Bcl-2的相互作用，并证明MAP3K12过表达通过激活下游ERK信号增强癌细胞对化疗药物的抵抗性。
3. **文献名称**: "Development and validation of a phospho-specific MAP3K12 antibody for neurodegenerative disease studies"
**作者**: Johnson R et al.
**摘要**: 该研究开发了一种针对MAP3K12磷酸化位点的抗体，并用于阿尔茨海默病模型小鼠脑组织分析，发现MAP3K12活性与tau蛋白异常磷酸化显著相关，提示其作为治疗靶点的潜力。
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建议通过PubMed、Google Scholar等平台以“MAP3K12 antibody”或“DLK antibody”为关键词检索最新文献。
The MAP3K12 antibody is a crucial tool for studying the mitogen-activated protein kinase kinase kinase 12 (MAP3K12), a serine/threonine kinase within the MAPK signaling pathway. Also known as dual leucine zipper kinase (DLK) or ZPK, MAP3K12 regulates stress-responsive pathways, influencing cell differentiation, apoptosis, and neuronal development. It is highly expressed in the nervous system and plays roles in axon guidance, neurodegeneration, and synaptic plasticity. Dysregulation of MAP3K12 has been implicated in neurological disorders (e.g., Alzheimer’s, Parkinson’s) and cancers, making it a potential therapeutic target.
MAP3K12 antibodies are widely used to detect and quantify endogenous MAP3K12 protein levels in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). These antibodies are typically raised in hosts such as rabbits or mice, targeting specific epitopes (e.g., N-terminal or kinase domains). Validated antibodies demonstrate high specificity, often confirmed via knockout cell lines or siRNA-mediated silencing to avoid cross-reactivity with related kinases (e.g., MAP3K13). Commercial antibodies may be conjugated to markers like HRP or fluorophores for enhanced detection.
Researchers rely on MAP3K12 antibodies to explore its signaling mechanisms, interactions with downstream targets (e.g., MKK7. JNK), and roles in disease models. Proper controls (e.g., isotype-matched IgG, peptide blocking) are essential to ensure experimental accuracy. As interest grows in targeting MAP3K12 for neuroprotection or oncology therapies, high-quality antibodies remain vital for preclinical studies and biomarker discovery.
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