| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/15-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | KHS; GCKR; KHS1; MAPKKKK5 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MAP4K5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAP4K5抗体的参考文献示例(注：以下内容为模拟示例，实际文献需根据具体数据库查询)：
1. **标题**：MAP4K5 regulates apoptosis via c-Jun phosphorylation in hepatocellular carcinoma
**作者**：Zhang L, et al.
**摘要**：本研究通过Western blot和免疫组化实验，利用MAP4K5抗体检测其在肝癌组织中的表达，发现MAP4K5通过磷酸化c-Jun调控肿瘤细胞凋亡，其高表达与患者预后不良相关。
2. **标题**：Role of MAP4K5 in autophagy under diabetic nephropathy conditions
**作者**：Wang Y, et al.
**摘要**：通过免疫沉淀和免疫荧光技术，使用MAP4K5抗体探究其在糖尿病肾病中的作用，结果表明MAP4K5通过mTOR通路抑制自噬，敲低后显著减轻肾脏细胞损伤。
3. **标题**：MAP4K5 modulates T-cell activation through JNK signaling
**作者**：Chen R, et al.
**摘要**：研究采用MAP4K5抗体进行流式细胞术和Western blot分析，发现MAP4K5通过激活JNK信号通路促进T细胞活化，为自身免疫疾病治疗提供潜在靶点。
4. **标题**：MAP4K5 inhibition attenuates cardiac hypertrophy in vivo
**作者**：Kim S, et al.
**摘要**：通过特异性抗体抑制MAP4K5活性，结合小鼠模型实验，证实MAP4K5参与心脏肥大的病理过程，其表达水平与心肌细胞肥大程度正相关。
(注：以上文献为示例性质，实际引用需核实具体来源及内容。)
The MAP4K5 (mitogen-activated protein kinase kinase kinase kinase 5) antibody is a research tool used to detect and study the MAP4K5 protein, a member of the Ste20 family of serine/threonine kinases. MAP4K5 plays a critical role in cellular signaling pathways, particularly those regulating stress responses, apoptosis, and metabolic homeostasis. It activates downstream kinases, including JNK (c-Jun N-terminal kinase) and p38 MAPK pathways, influencing processes like inflammation, cell proliferation, and insulin signaling.
Antibodies targeting MAP4K5 are widely employed in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate its expression, localization, and interaction partners in various tissues and disease models. Research has linked MAP4K5 dysregulation to conditions such as cancer, neurodegenerative disorders, and metabolic syndromes, making it a potential therapeutic target.
These antibodies are typically validated for specificity and sensitivity using knockout controls or siRNA-mediated knockdown. Commercial MAP4K5 antibodies are available from multiple suppliers, often raised in hosts like rabbits or mice, with options targeting specific epitopes or post-translational modifications. Their applications span basic research to drug discovery, aiding in elucidating MAP4K5's role in cellular mechanisms and disease progression.
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