| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ZRF; MTF-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MTF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与MTF1抗体相关的代表性文献，信息已简化整理：
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1. **文献名称**: "MTF-1 mediates hypoxia-induced transcriptional activation of miR-210 in cancer cells"
**作者**: Zhang Y et al.
**摘要**: 研究利用MTF1抗体(ChIP实验)证明MTF1在低氧条件下直接结合miR-210启动子，调控其表达，促进肿瘤细胞存活。揭示了MTF1在肿瘤微环境中的新调控机制。
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2. **文献名称**: "Metal-responsive transcription factor 1 regulates synaptic zinc levels and neuronal senescence"
**作者**: Smith J et al.
**摘要**: 通过MTF1抗体进行免疫印迹和免疫组化分析，发现MTF1缺失导致神经元锌稳态失衡，加速衰老进程。研究提示MTF1在神经退行性疾病中可能起保护作用。
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3. **文献名称**: "A novel role for MTF-1 in toll-like receptor signaling and innate immunity"
**作者**: Lee S & Kim DH
**摘要**: 使用MTF1抗体进行免疫共沉淀实验，发现MTF1与TLR4信号通路相互作用，调控巨噬细胞炎症因子释放。为金属代谢与免疫反应的交叉调控提供了证据。
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**备注**：以上文献为模拟示例，实际研究需通过PubMed或Web of Science检索关键词"MTF1 antibody"或"MTF1 ChIP"获取具体文献。建议优先选择近五年内的高分期刊论文(如《Nature Cell Biology》《EMBO Journal》等)以获取最新研究进展。
MTF1 (Metal-regulatory transcription factor 1) is a zinc-regulated transcription factor that plays a critical role in cellular responses to heavy metal stress and oxidative damage. It binds to metal-responsive elements (MREs) in the promoter regions of target genes, such as metallothioneins (MTs), to regulate their expression. MTF1 is activated under conditions of elevated intracellular zinc, cadmium, or other stressors, mediating detoxification pathways and maintaining metal homeostasis. Structurally, it contains six Cys2-His2 zinc fingers for DNA binding and distinct domains for nuclear localization and transcriptional activation.
MTF1 antibodies are essential tools for studying its expression, localization, and function in various biological contexts. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect endogenous MTF1 levels, monitor its translocation from the cytoplasm to the nucleus under stress, and identify target gene interactions. Research involving MTF1 antibodies has provided insights into its role in development, cancer (e.g., regulating hypoxia-responsive genes), and neurodegenerative diseases linked to metal dysregulation. Additionally, they aid in exploring MTF1's interplay with signaling pathways like the Keap1-Nrf2 system, highlighting its broader implications in redox balance and cytoprotection.
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