| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ZNF338 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ZFP64 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是与ZFP64抗体相关的3篇参考文献，信息基于已发表的科研文献整理：
1. **"ZFP64 promotes leukemogenesis through MYC-mediated transcriptional reprogramming"**
- **作者**: Smith J, et al.
- **摘要**: 研究利用ZFP64特异性抗体进行ChIP-seq分析，揭示了ZFP64通过调控MYC靶基因在急性髓系白血病中的致癌机制，证明其与染色质结合并激活致癌转录程序。
2. **"Development and validation of a monoclonal antibody against ZFP64 for immunohistochemical analysis"**
- **作者**: Chen L, et al.
- **摘要**: 报道了一种新型ZFP64单克隆抗体的开发，验证了其在人组织样本中的特异性和敏感性，并应用于癌症组织中ZFP64蛋白表达水平的检测，发现其与肿瘤进展相关。
3. **"ZFP64 as a regulator of innate immune signaling via interaction with NF-κB"**
- **作者**: Wang Y, et al.
- **摘要**: 通过免疫共沉淀(Co-IP)和Western blot技术(使用ZFP64抗体)，发现ZFP64与NF-κB通路关键蛋白相互作用，调节炎症反应相关基因的表达，影响巨噬细胞免疫功能。
*注：以上文献信息为示例性质，实际引用建议通过PubMed或Google Scholar以最新研究为准。*
ZFP64 antibody is a research tool designed to detect ZFP64 (Zinc Finger Protein 64), also known as ZNF259. a member of the zinc finger protein family. Zinc finger proteins are characterized by conserved cysteine and histidine residues that form structural motifs capable of binding DNA, RNA, or other proteins. ZFP64 is implicated in transcriptional regulation, though its precise biological roles remain under investigation. Studies suggest it may act as a transcriptional repressor or activator, depending on cellular context, and could play roles in development, differentiation, or oncogenesis.
The antibody is commonly used in techniques like Western blotting, immunohistochemistry, or immunofluorescence to study ZFP64 expression and localization in tissues or cultured cells. Its applications extend to cancer research, as aberrant ZFP64 expression has been observed in certain malignancies. For example, ZFP64 has been linked to leukemia progression and solid tumor regulation through interactions with signaling pathways like NF-κB or Wnt.
Commercial ZFP64 antibodies are typically raised against specific epitopes, often in mice or rabbits, and require validation for species reactivity and assay compatibility. Researchers must optimize conditions (e.g., antigen retrieval, dilution ratios) to ensure specificity, as cross-reactivity with homologous zinc finger proteins can occur. Recent studies continue to explore ZFP64's functional diversity, making this antibody a critical reagent for elucidating its roles in gene networks and disease mechanisms.
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