关键信息
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基因名
SKP1-SKP2
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简介
The SKP1 protein is a component of the SCF ubiquitin ligase complex and targets cell cycle progression and signal transduction. SKP1/SKP2 Protein, Human (Active) is the recombinant human-derived SKP1/SKP2, expressed by E. coli, with tag-free.
- 应用
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别名
EMC19; OCP2; SKP1A; TCEB1L
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种属
Human
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P63208 (M1-K163)&Q13309-1
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表达区间
P63208 (M1-K163)&Q13309-1 (R89-L424)
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SKP1 and SKP2 are crucial components of the SCF (SKP1-Cullin-F-box) E3 ubiquitin ligase complex, which plays a significant role in the regulation of protein degradation pathways through the ubiquitin-proteasome system. SKP1 serves as an adaptor protein that links F-box proteins, which recognize specific substrates, to the Cullin scaffold, thus facilitating the ubiquitination process. SKP2, an F-box protein, is known for its role in the cell cycle regulation by targeting the cyclin-dependent kinase inhibitor p27 for degradation, thereby promoting cell cycle progression and cellular proliferation. Dysregulation of SKP2 has been implicated in various cancers, making it a valuable target for therapeutic interventions. The study and characterization of SKP1-SKP2 recombinant proteins are essential for understanding the molecular mechanisms underlying their function in ubiquitination and cell cycle control. Researchers employ techniques like protein expression and purification to investigate the structural properties and interactions of these proteins, which can reveal potential drug targets or inform the development of inhibitors that disrupt abnormal protein degradation pathways in cancer. Overall, the exploration of SKP1-SKP2 recombination provides insights into the complex regulatory networks governing cellular processes and has significant implications for cancer therapy and other diseases related to protein homeostasis.
