| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BBH; BBOX; G-BBH; gamma-BBH |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human BBOX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于BBOX1抗体的3篇参考文献摘要整理：
1. **文献名称**：The role of BBOX1 in carnitine biosynthesis and mitochondrial function
**作者**：Li Y, et al.
**摘要**：本研究利用BBOX1特异性抗体，通过Western blot和免疫组化技术验证了BBOX1蛋白在肝脏组织中的高表达，并揭示其在调控线粒体脂肪酸氧化中的关键作用。抗体特异性通过siRNA敲低实验验证。
2. **文献名称**：BBOX1 as a novel biomarker in diabetic nephropathy progression
**作者**：Zhang H, et al.
**摘要**：文章通过BBOX1抗体检测肾小球细胞中该酶的表达水平，发现其与糖尿病肾病患者的氧化应激程度呈正相关。抗体应用于流式细胞术和免疫荧光，证实了其在疾病模型中的诊断潜力。
3. **文献名称**：Regulation of BBOX1 by microRNA-29 in cardiovascular diseases
**作者**：Wang X, et al.
**摘要**：研究使用BBOX1抗体进行心肌细胞蛋白定量，结合qPCR分析，发现miR-29通过靶向抑制BBOX1影响心肌能量代谢。抗体特异性通过过表达/敲除实验交叉验证。
注：以上文献信息为基于公开研究方向的模拟概括，实际引用需核对具体文献来源。
BBOX1 (γ-butyrobetaine hydroxylase) is a key enzyme in the biosynthesis of L-carnitine, a molecule essential for fatty acid metabolism. It catalyzes the final step in carnitine synthesis by hydroxylating γ-butyrobetaine to form L-carnitine, primarily in the liver and kidneys. BBOX1's role in energy metabolism links it to various physiological and pathological processes, including cardiac function, muscle performance, and metabolic disorders.
Antibodies targeting BBOX1 are widely used in research to study its expression, localization, and regulation. These antibodies enable detection of BBOX1 protein levels in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Studies employing BBOX1 antibodies have explored its involvement in diseases such as diabetes, obesity, and cancer, where altered carnitine metabolism may contribute to pathogenesis. Additionally, research has investigated BBOX1's potential as a biomarker or therapeutic target in metabolic syndromes.
Commercial BBOX1 antibodies are typically raised against specific epitopes of human BBOX1. with validation in common experimental models. Researchers often verify antibody specificity using knockout controls or siRNA-mediated gene silencing. Recent work also examines BBOX1's interaction networks and post-translational modifications, facilitated by immunoprecipitation-competent antibodies. As interest in metabolic reprogramming grows, BBOX1 antibodies remain crucial tools for understanding cellular energy regulation mechanisms.
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