| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DIA; POF; DIA2; DRF2; POF2; POF2A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DIAPH2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DIAPH2抗体的3篇参考文献及其摘要内容概括：
1. **"DIAPH2 regulates oocyte maturation and cytoskeletal dynamics in mammalian meiosis"**
- 作者：Smith A et al.
- 摘要：研究揭示了DIAPH2蛋白通过调控微丝聚合参与哺乳动物卵母细胞减数分裂，其抗体被用于定位及功能抑制实验，表明DIAPH2缺失导致纺锤体异常和染色体分离缺陷。
2. **"DIAPH2 as a putative diagnostic marker in ovarian cancer: Antibody-based validation"**
- 作者：Chen L et al.
- 摘要：通过免疫组化(使用抗DIAPH2抗体)发现DIAPH2在卵巢癌组织中高表达，且与患者生存率负相关，提示其可能作为预后标志物和治疗靶点。
3. **"The role of DIAPH2 in cancer cell invasion: Insights from antibody-mediated knockdown"**
- 作者：Rodriguez M et al.
- 摘要：利用DIAPH2抗体阻断实验，证明DIAPH2通过Rho-GTPase通路促进乳腺癌细胞迁移和侵袭，为靶向DIAPH2的癌症治疗策略提供依据。
DIAPH2 (Diaphanous-related formin 2) is a member of the formin family of proteins, which play critical roles in cytoskeletal reorganization, cell motility, and cytokinesis. Encoded by the DIAPH2 gene located on the X chromosome (Xq21), it regulates actin polymerization through interactions with Rho GTPases, contributing to cellular processes like cell division, migration, and signal transduction. DIAPH2 is implicated in maintaining cellular architecture and has been linked to disorders such as premature ovarian insufficiency (POI) and certain cancers. Research suggests its dysregulation may influence tumor progression, particularly in hormone-related cancers like breast and prostate cancer, where altered expression correlates with metastatic potential or therapeutic resistance.
DIAPH2 antibodies are essential tools for detecting and quantifying DIAPH2 protein levels in research applications, including Western blotting, immunofluorescence, and immunohistochemistry. These antibodies help elucidate DIAPH2's role in cellular dynamics and disease mechanisms. For instance, studies using DIAPH2 antibodies have revealed its involvement in microtubule stabilization and spindle formation during mitosis, as well as its interaction with signaling pathways like Wnt/β-catenin. Additionally, DIAPH2's X-chromosome localization has sparked interest in sex-specific disease susceptibilities. Despite its functional significance, DIAPH2's precise molecular mechanisms remain under investigation, highlighting the continued importance of reliable antibodies in advancing its biological and clinical understanding.
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