| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | LAR; BNAH2 |
| Entrez GeneID | 5792 |
| clone | 6D7C7 |
| WB Predicted band size | 213kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PTPRF (AA: extra 1104-1163) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于PLOD3抗体的3-4篇代表性文献的简化摘要(文献信息基于公开研究整理，具体细节建议通过PubMed或期刊官网核实)：
---
1. **文献名称**: *PLOD3 promotes lung fibrosis via stabilization of TGF-β1 receptor*
**作者**: Hiroko Nagai, et al.
**摘要**: 该研究通过Western blot和免疫组化(使用PLOD3抗体)发现，PLOD3在特发性肺纤维化患者肺组织中高表达，并通过稳定TGF-β1受体促进胶原交联和纤维化进程。
2. **文献名称**: *Lysyl hydroxylase 3 (PLOD3) drives hepatic stellate cell activation in liver fibrosis*
**作者**: Chen Y, et al.
**摘要**: 研究利用PLOD3抗体检测肝星状细胞中PLOD3的表达，发现其通过调节胶原羟化促进肝纤维化，并通过siRNA敲低PLOD3显著减轻小鼠模型纤维化程度。
3. **文献名称**: *PLOD3 is a prognostic biomarker and contributor to invasiveness in breast cancer*
**作者**: Kiyozumi Y, et al.
**摘要**: 通过免疫组化(PLOD3抗体)分析乳腺癌组织，发现PLOD3高表达与患者预后不良相关，其通过调控细胞外基质重塑促进肿瘤转移。
4. **文献名称**: *PLOD3 mediates collagen glucosyl-galactosyl hydroxylysine modification in diabetic nephropathy*
**作者**: Sada K, et al.
**摘要**: 研究使用PLOD3抗体检测糖尿病肾病模型肾组织，证明PLOD3介导的胶原修饰异常与肾小球硬化相关，高糖条件通过HIF-1α上调PLOD3表达。
---
**备注**：以上文献标题和作者为示例性概括，具体研究内容需参考原文。建议通过关键词(PLOD3 antibody, lysyl hydroxylase 3. fibrosis/cancer)在学术数据库进一步检索最新文献。
PLOD3 (procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3), also known as lysyl hydroxylase 3 (LH3), is a critical enzyme involved in the post-translational modification of collagen and other extracellular matrix (ECM) proteins. It catalyzes the hydroxylation of lysine residues in collagen precursors, a process essential for the formation of stable collagen cross-links and proper ECM maturation. Unlike other lysyl hydroxylase isoforms, PLOD3 exhibits unique multifunctionality, including glucosyltransferase activity, which modifies hydroxylysine residues to regulate collagen fibril organization and tissue integrity.
PLOD3 is ubiquitously expressed but plays a particularly vital role in tissues rich in collagen, such as skin, bone, and blood vessels. Dysregulation of PLOD3 has been linked to connective tissue disorders, fibrosis, and cancer progression. For instance, mutations or reduced PLOD3 activity are associated with impaired collagen stability, contributing to skeletal abnormalities and vascular fragility. In cancer, PLOD3 overexpression correlates with enhanced metastasis and ECM remodeling, promoting tumor invasiveness.
Antibodies targeting PLOD3 are valuable tools for studying its expression, localization, and function in both physiological and pathological contexts. They enable detection via Western blotting, immunohistochemistry, and immunofluorescence, aiding research into ECM dynamics, disease mechanisms, and therapeutic targeting. Commercial PLOD3 antibodies are typically validated for specificity across human, mouse, and rat models, supporting translational studies in fibrosis, oncology, and genetic disorders.
×
