| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/5000 | Human,Mouse,Rat |
| Aliases | CK5, K5, DDD, EBS2, KRT5A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human KRT5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KRT5抗体的3篇代表性文献的简要总结(文献信息为示例，非真实发表内容)：
1. **《KRT5 as a Biomarker in Basal-like Breast Cancer: Validation of Antibody Specificity》**
- **作者**: Smith et al. (2020)
- **摘要**: 本研究验证了KRT5抗体在基底样乳腺癌分型中的应用，通过免疫组化(IHC)和Western blot证明其特异性。结果显示KRT5高表达与基底样亚型和较差预后相关，提示其可作为分子分型的可靠标志物。
2. **《KRT5 Expression in Cutaneous Squamous Cell Carcinoma: Diagnostic Utility》**
- **作者**: Johnson et al. (2018)
- **摘要**: 探讨KRT5抗体在皮肤鳞状细胞癌(cSCC)中的诊断价值。研究发现，KRT5在肿瘤基底层的强表达可有效区分cSCC与其他皮肤病变，支持其作为组织病理学诊断工具的应用。
3. **《Role of KRT5 in Epithelial Stem Cell Niches: Insights from Antibody-based Staining》**
- **作者**: Lee et al. (2019)
- **摘要**: 利用KRT5抗体定位表皮干细胞，发现其在毛囊和表皮基底层中的特异性表达，证实KRT5是维持上皮干细胞特性的关键标记物，为组织再生研究提供依据。
注：以上文献为示例，实际引用需查询真实数据库(如PubMed)获取具体信息。
Keratin 5 (KRT5) is a type II intermediate filament protein encoded by the *KRT5* gene, primarily expressed in basal keratinocytes of stratified epithelia, such as skin, breast, and other epithelial tissues. As a key structural component of the cytoskeleton, it pairs with type I keratins (e.g., KRT14) to form networks that maintain cellular integrity, mechanical resilience, and tissue homeostasis. KRT5 is a well-established marker for basal epithelial cells and progenitor cells, playing roles in cell differentiation, wound healing, and stress response.
Antibodies targeting KRT5 are widely used in research and diagnostics. In pathology, KRT5 antibodies help identify basal cell carcinomas, squamous cell carcinomas, and certain subtypes of breast cancer (e.g., basal-like breast cancer). They are also employed to study benign skin disorders, such as epidermolysis bullosa simplex, where *KRT5* mutations disrupt keratin filament assembly. Additionally, these antibodies aid in distinguishing epithelial lineages in developmental biology and cancer research.
Commercially available KRT5 antibodies are validated for techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting. Their specificity and reliability make them critical tools for characterizing tissue architecture, tumor classification, and understanding epithelial-mesenchymal transitions. Recent studies also explore KRT5's potential as a prognostic indicator or therapeutic target in malignancies, underscoring its biomedical relevance.
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