| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/10000 | Human,Mouse,Rat |
| Aliases | BARK1; ADRBK1; BETA-ARK1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GRK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与GRK2抗体相关的文献概述(文献信息为模拟示例，建议通过学术数据库核实具体内容)：
1. **"GRK2 inhibition in heart failure: a novel therapeutic approach"**
*作者：Koch WJ et al. (2003)*
摘要：研究通过特异性GRK2抗体检测心力衰竭模型中GRK2蛋白表达上调，证实其与β-肾上腺素受体信号异常相关，为抗体在心血管疾病机制研究中的应用提供依据。
2. **"Role of GRK2 in immune cell signaling and inflammation"**
*作者：Pitcher JA et al. (2012)*
摘要：利用GRK2抗体进行免疫印迹和免疫荧光实验，揭示GRK2在巨噬细胞中的表达动态变化及其对炎症因子信号通路的调控作用，提示抗体在免疫学研究中的工具价值。
3. **"GRK2 as a potential biomarker in breast cancer progression"**
*作者：Penela P et al. (2016)*
摘要：通过免疫组化分析临床样本，发现GRK2抗体标记的蛋白水平与乳腺癌转移正相关，表明其可作为预后标志物及治疗靶点。
**注**：以上为简化示例，实际文献需通过PubMed/Google Scholar检索关键词“GRK2 antibody”、“GRK2 immunohistochemistry”或结合具体研究领域(如心血管、肿瘤)获取准确信息。建议优先选择近5年高被引论文或权威期刊研究。
GRK2 (G protein-coupled receptor kinase 2) is a serine/threonine kinase primarily involved in regulating G protein-coupled receptor (GPCR) signaling. It phosphorylates agonist-activated GPCRs, promoting their desensitization and internalization via interactions with β-arrestin. GRK2 is ubiquitously expressed and plays critical roles in cardiovascular, immune, and neurological systems. Dysregulation of GRK2 has been linked to pathologies such as heart failure, hypertension, cancer, and metabolic disorders. For instance, elevated GRK2 levels in heart failure impair β-adrenergic receptor signaling, exacerbating cardiac dysfunction.
GRK2 antibodies are essential tools for studying its expression, localization, and function in physiological and disease contexts. These antibodies enable detection through techniques like Western blotting, immunohistochemistry, and flow cytometry. Specific monoclonal or polyclonal antibodies target distinct epitopes, with validation often including knockout controls to confirm specificity. Research using GRK2 antibodies has revealed its interactions with non-GPCR substrates (e.g., PI3K, HDAC6) and roles in cell migration, inflammation, and insulin resistance.
Therapeutic targeting of GRK2 is an emerging area, with inhibitors under investigation for cardiovascular and oncological applications. Antibodies against GRK2 also aid in biomarker studies, correlating its expression levels with disease progression or therapeutic responses. Overall, GRK2 antibodies are pivotal in unraveling the kinase's multifaceted roles and advancing translational research.
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