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Rabbit Polyclonal GCH1 Antibody

  • 中文名: GCH1抗体
  • 别    名: GCH, DYT5, DYT14, DYT5a, GTPCH1, HPABH4B, GTP-CH-1
货号: IPDX10938
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/5-1/20 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/1000-1/5000 Human,Mouse,Rat

产品详情

AliasesGCH, DYT5, DYT14, DYT5a, GTPCH1, HPABH4B, GTP-CH-1
WB Predicted band size28 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthetic peptide of human GCH1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于GCH1抗体的参考文献示例(注:以下内容为模拟示例,实际文献需通过学术数据库查询验证):

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1. **文献名称**: *"GCH1 Antibody Specificity and Application in Neuropathic Pain Models"*

**作者**: Tegeder, I. et al.

**摘要**: 该研究通过Western blot和免疫组化验证了GCH1抗体的特异性,发现其在背根神经节中的表达与神经性疼痛模型中BH4水平相关。抗体成功检测到GCH1蛋白在炎症条件下的表达上调,支持其在疼痛机制研究中的应用。

2. **文献名称**: *"Endothelial Dysfunction and GCH1 Downregulation in Cardiovascular Disease"*

**作者**: Antoniades, C. et al.

**摘要**: 研究利用GCH1抗体分析人内皮细胞中GCH1蛋白的表达,发现其下调导致BH4合成减少,引发一氧化氮合酶解偶联,与动脉粥样硬化和高血压相关。抗体特异性通过siRNA敲低实验验证。

3. **文献名称**: *"GCH1 Expression in Dopaminergic Neurons: Implications for Parkinson’s Disease"*

**作者**: Zhao, Y. et al.

**摘要**: 通过免疫荧光和GCH1抗体标记,研究发现帕金森病模型中黑质区GCH1蛋白水平显著降低,提示BH4代谢异常可能参与多巴胺能神经元退化,为疾病机制提供新见解。

4. **文献名称**: *"Development of a Polyclonal GCH1 Antibody for Immunohistochemical Analysis"*

**作者**: Daly, S.B. et al.

**摘要**: 文章描述了一种兔源多克隆GCH1抗体的制备与验证,该抗体能特异性识别人类和小鼠组织中的GCH1蛋白,适用于冰冻切片和石蜡切片的检测,为临床病理研究提供工具。

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**提示**:实际文献需通过PubMed、Google Scholar等平台检索关键词“GCH1 antibody”“GCH1 immunoblotting”或结合具体研究领域(如疼痛、心血管疾病)进一步筛选。部分研究可能侧重于抗体开发或特定疾病的GCH1蛋白检测应用。

背景信息

The GCH1 antibody targets GTP cyclohydrolase 1 (GCH1), a rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis. BH4 is a critical cofactor for neurotransmitters (e.g., dopamine, serotonin) and nitric oxide synthesis, linking GCH1 to neurological and vascular functions. GCH1 mutations cause hereditary dopa-responsive dystonia (DRD), characterized by movement disorders and treatable with L-DOPA. In research, GCH1 antibodies are used to study protein expression in tissues or cells via techniques like Western blotting, immunohistochemistry, or immunofluorescence. These tools help explore GCH1's role in neuroprotection, pain regulation, cardiovascular health, and immune responses. Dysregulated GCH1/BH4 pathways are implicated in neurodegenerative diseases (e.g., Parkinson’s), chronic pain syndromes, hypertension, and endothelial dysfunction. Antibody-based studies also investigate GCH1 as a biomarker or therapeutic target, particularly in conditions involving oxidative stress or inflammation. Commercially available GCH1 antibodies are typically validated in human, mouse, or rat models, with specificity confirmed through knockout controls. Ongoing research aims to clarify GCH1's dual roles in physiological homeostasis and disease pathogenesis, highlighting its potential in precision medicine and drug development.

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