| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | ASK1, MEKK5, MAPKKK5 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MAP3K5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAP3K5(ASK1)抗体的3篇参考文献示例(内容为模拟概括，非真实文献)：
1. **文献名称**：*ASK1 is essential for JNK/SAPK activation by TRAF2*
**作者**：Tobiume K, et al.
**摘要**：该研究揭示了MAP3K5(ASK1)在TNF-α诱导的JNK信号通路中的核心作用，通过抗体检测ASK1蛋白表达及磷酸化水平，证实其与TRAF2的相互作用介导了细胞凋亡。
2. **文献名称**：*Role of ASK1 in oxidative stress-induced neuronal apoptosis*
**作者**：Nishitoh H, et al.
**摘要**：利用MAP3K5特异性抗体进行免疫印迹和免疫共沉淀实验，证明氧化应激通过激活ASK1-p38/JNK通路触发神经元凋亡，抗体阻断实验进一步验证其功能。
3. **文献名称**：*ASK1 inhibition attenuates cardiac hypertrophy in vivo*
**作者**：Izumiya Y, et al.
**摘要**：研究通过抗MAP3K5抗体检测心脏组织中ASK1的活性变化，发现抑制ASK1可减轻压力超负荷引起的心肌肥厚，提示其作为治疗靶点的潜力。
(注：以上为简化示例，实际文献需通过PubMed等数据库检索确认。)
The MAP3K5 antibody targets mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as apoptosis signal-regulating kinase 1 (ASK1). MAP3K5 is a serine/threonine kinase within the MAPK signaling cascade, playing a pivotal role in cellular responses to stress, inflammation, apoptosis, and differentiation. It activates downstream kinases like JNK and p38 by phosphorylating MAP2Ks (e.g., MKK4/7. MKK3/6) in response to oxidative stress, cytokines, or DNA damage. Dysregulation of MAP3K5 is linked to pathologies such as cancer, neurodegenerative disorders, cardiovascular diseases, and metabolic syndromes.
Antibodies against MAP3K5 are essential tools for studying its expression, localization, and activation. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) to assess protein levels, phosphorylation status (e.g., Thr845/838), or interactions with partners like TRAF2/6 in specific tissues or disease models. High-quality MAP3K5 antibodies are validated for specificity using knockout controls or siRNA knockdown.
Research employing MAP3K5 antibodies has revealed its dual role in apoptosis and survival, influenced by binding partners such as thioredoxin (which inhibits its activity under redox-balanced conditions). These studies highlight its potential as a therapeutic target, with inhibitors explored for conditions like fibrosis and neurodegeneration. Reliable antibodies remain critical for unraveling MAP3K5's context-dependent signaling mechanisms and therapeutic applications.
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