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产品描述：猪心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的cTn-I含量。cTn-I（心肌肌钙蛋白I）是一种心肌细胞特有的调节蛋白，在心肌损伤时释放进入血液，成为诊断心肌损伤的重要标志物。其研究机制涉及心肌损伤时cTn-I的释放和检测，对于早期诊断心肌梗死等心血管疾病具有重要意义。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域中心肌损伤相关机制研究；适用于动物模型中心脏疾病研究、心血管药物开发中的药效评估，以及体外实验中心肌细胞损伤模型的生物标志物检测，为科研人员提供稳定可靠的心肌损伤动态监测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：cTnⅠ
Uniprot No.：I3LLI0
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of pig cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-101 | ||||
| EDTA plasma (n=4) | 95 | 86-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10001.8151.8661.8411.703 5001.6521.7131.6831.545 2501.3471.4011.3741.236 1251.0060.9570.9820.844 62.50.7430.7250.7340.596 31.250.5140.5080.5110.373 15.60.3240.3080.3160.178 00.1360.1390.138 | ||||||
货期：3-5 working days
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