首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠细胞色素C(Cyt-C)酶联免疫试剂盒 双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYCS含量。CYCS即细胞色素 c，是线粒体呼吸链的重要组成部分。它在细胞呼吸和能量代谢中起关键作用。研究发现，CYCS还参与细胞凋亡机制，当细胞受到凋亡信号刺激时，它会从线粒体释放到胞质中，激活凋亡相关酶，引发细胞程序性死亡。试剂盒检测范围为34.3 pg/mL-2200 pg/mL，适用于科研场景下细胞凋亡机制及线粒体功能相关研究；可为神经退行性疾病模型、心肌缺血再灌注损伤、肿瘤治疗响应等基础研究提供关键数据；适用于实验室规模的高通量筛选或小样本量研究，尤其在药物干预后线粒体功能评估、基因编辑模型验证等场景中具有重要应用价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cycs ELISA Kit; Cytochrome c ELISA Kit; somatic ELISA Kit
缩写：CYCS
Uniprot No.：P62897
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：34.3 pg/mL-2200 pg/mL
灵敏度：8.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-97 | ||||||
| 1:2 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of mouse Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 95-105 | |||||
| EDTA plasma (n=4) | 87 | 83-91 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected22002.3522.5122.4322.24011001.6151.5781.5971.4055501.0731.0231.0480.8562750.6390.6810.6600.468137.50.4610.4890.4750.28368.750.3870.3740.3810.18934.30.3180.3050.3120.12000.1940.1890.192 | |||||||
货期：3-5 working days
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