首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠芳香酶酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYP19A1含量。CYP19A1又称芳香化酶，是催化雄激素转化为雌激素的限速酶。其作为重要药物靶点，在乳腺癌等激素依赖性疾病中高度表达，与肿瘤生长、增殖密切相关。研究机制主要围绕抑制其活性，减少雌激素合成，以此达到治疗疾病的目的。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于研究芳香酶在激素代谢调控中的作用，广泛参与生殖系统发育、内分泌平衡及性激素依赖性疾病的分子机制研究，可为生殖生物学、肿瘤微环境分析及内分泌疾病模型构建提供可靠数据支持，例如用于卵巢功能评估、激素代谢异常研究或药物干预下酶活性变化的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cyp19a1 ELISA kit; Arom ELISA kit; Cyp19Aromatase ELISA kit; EC 1.14.14.14 ELISA kit; CYPXIX ELISA kit; Cytochrome P-450AROM ELISA kit; Cytochrome P450 19A1 ELISA kit; Estrogen synthase ELISA kit
缩写：CYP19A1
Uniprot No.：P28649
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ARO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-96 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-104 | |||||
回收率：
| The recovery of mouse ARO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 92 | 86-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 4002.2582.1122.1852.009 2001.6441.6951.6701.494 1001.0281.1121.0700.894 500.5880.6040.5960.420 250.3340.3580.3460.170 12.50.2490.2570.2530.077 6.250.2140.2260.2200.044 00.1810.1710.176 | ||||||
货期：3-5 working days
×
