首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人细胞因子样蛋白1(CYTL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYTL1含量。CYTL1是一种小分子量的分泌蛋白，最初在CD34+细胞中发现。研究发现，CYTL1在细胞内具有新型抑癌因子特征，通过稳定NDUFV1抑制乳腺癌代谢重编程，其作用机制涉及与MDM2竞争性结合在NDUFV1的1-80结构域，从而干扰NDUFV1的泛素化降解，实现稳态的OXPHOS代谢。试剂盒检测范围为0.156 ng/mL-10 ng/mL，可广泛应用于细胞因子调控机制研究、疾病动物模型中的蛋白表达动态分析，或体外培养细胞上清液中CYTL1分泌水平的定量评估，为探索其生物学功能及信号通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C17 ELISA Kit; C4orf4 ELISA Kit; CYTL1 ELISA Kit; CYTL1_HUMAN ELISA Kit; Cytokine like 1 ELISA Kit; Cytokine like protein C17 ELISA Kit; Cytokine-like protein 1 ELISA Kit; Protein C17 ELISA Kit
缩写：CYTL1
Uniprot No.：Q9NRR1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYTL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-97 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 88-97 | ||||||
回收率：
| The recovery of human CYTL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-99 | |||||
| EDTA plasma (n=4) | 103 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 102.0082.1022.0551.877 51.7351.7971.7661.588 2.51.4081.3691.3891.211 1.251.1211.0681.0950.917 0.6250.7370.7280.7330.555 0.3120.4880.4680.4780.300 0.1560.3360.3310.3340.156 00.1780.1780.178 | |||||||
货期：3-5 working days
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