| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | TORC2; TORC-2 |
| WB Predicted band size | 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CRTC2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于CRTC2抗体的3篇参考文献的简要列举：
1. **文献名称**: *CREB and CRTC2 Cooperatively Regulate Hepatic Gluconeogenesis*
**作者**: Koo SH, et al.
**摘要**: 该研究利用CRTC2抗体通过免疫共沉淀和染色质免疫沉淀技术，揭示了CRTC2与CREB在肝脏糖异生中的协同调控机制，发现胰岛素通过AKT信号通路抑制CRTC2的核转位。
2. **文献名称**: *The CREB coactivator CRTC2 links hepatic ER stress and fasting gluconeogenesis*
**作者**: Wang Y, et al.
**摘要**: 研究使用CRTC2抗体进行Western blot和免疫荧光分析，证明内质网应激通过PERK-eIF2α信号激活CRTC2.促进肝脏糖异生关键基因表达，与2型糖尿病病理相关。
3. **文献名称**: *CRTC2 Is Required for β-cell Survival and Insulin Secretion*
**作者**: Screaton RA, et al.
**摘要**: 通过CRTC2抗体检测胰岛β细胞中的蛋白表达，发现CRTC2通过调控Mcl-1等抗凋亡基因维持β细胞存活，其功能异常可能导致糖尿病发生。
*注：以上文献信息为示例性质，实际引用时请核对期刊名称、作者及年份的准确性。*
The CRTC2 (CREB-regulated transcription coactivator 2) antibody is a key tool for studying the regulatory role of CRTC2 in cellular signaling and metabolic pathways. CRTC2. also known as TORC2. is a transcriptional coactivator that modulates CREB (cAMP response element-binding protein)-dependent gene expression. It plays a critical role in glucose homeostasis, energy balance, and stress responses by integrating signals from pathways such as insulin, glucagon, and AMP-activated protein kinase (AMPK). Under basal conditions, CRTC2 is phosphorylated and sequestered in the cytoplasm; upon dephosphorylation (e.g., via calcineurin), it translocates to the nucleus to enhance CREB-mediated transcription of target genes like PEPCK and G6Pase, which are involved in gluconeogenesis.
The CRTC2 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect CRTC2 expression, localization, and post-translational modifications. It is particularly valuable in metabolic research, such as investigating insulin resistance, diabetes, or hepatic glucose overproduction. Studies also utilize this antibody to explore CRTC2's involvement in cancer, circadian rhythm regulation, and neurodegenerative diseases. Validated antibodies are essential to ensure specificity, as CRTC2 shares homology with other CRTC family members (CRTC1/3). Researchers often verify antibody performance using knockout controls or siRNA-mediated CRTC2 knockdown. Commercial CRTC2 antibodies are typically raised against unique peptide sequences within the N- or C-terminal regions of human or mouse CRTC2.
×
