| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SMAP; p120; SMAP2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human BRD8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与BRD8抗体相关的文献示例(内容基于真实研究概括，具体文献标题和作者为示例性信息)：
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1. **文献名称**: *"BRD8 maintains glioblastoma through epigenetic regulation of histone H3 lysine 27 acetylation"*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用BRD8特异性抗体进行染色质免疫沉淀(ChIP)，揭示BRD8通过维持组蛋白H3K27乙酰化水平调控胶质母细胞瘤细胞的增殖和存活，为靶向BRD8的表观遗传治疗提供依据。
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2. **文献名称**: *"Development of a monoclonal antibody targeting the bromodomain of BRD8 for cancer biomarker discovery"*
**作者**: Smith RJ, et al.
**摘要**: 文章报道了一种新型抗BRD8溴结构域的单克隆抗体的开发与验证。该抗体在乳腺癌组织中的高特异性检测表明，BRD8可能作为癌症预后标志物，并参与肿瘤代谢重编程。
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3. **文献名称**: *"BRD8 interacts with SWI/SNF complex to regulate DNA damage repair in ovarian cancer"*
**作者**: Lee H, et al.
**摘要**: 通过免疫共沉淀(Co-IP)和Western blot分析，研究发现BRD8抗体可特异性识别其与SWI/SNF染色质重塑复合物的相互作用，并证明BRD8缺失导致卵巢癌细胞对DNA损伤药物的敏感性增加。
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(注：以上文献为示例性概括，实际研究中请通过PubMed或Google Scholar检索具体文献。)
The BRD8 antibody is a research tool designed to target the Bromodomain-containing protein 8 (BRD8), a member of the bromodomain and extra-terminal (BET) family. BRD8. also known as SMAP or PP9453. plays a role in chromatin remodeling and transcriptional regulation by recognizing acetylated lysine residues on histones through its bromodomain. It interacts with chromatin-modifying complexes, such as the SWI/SNF and NuA4/TIP60 complexes, influencing gene expression, DNA repair, and cell cycle progression. Dysregulation of BRD8 has been implicated in cancers, neurodevelopmental disorders, and viral infection responses.
BRD8 antibodies are widely used in studies to detect protein expression, localization, and function via techniques like Western blotting, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation (ChIP). These antibodies help elucidate BRD8's role in epigenetic mechanisms and disease pathways. Specific clones are validated for cross-reactivity, sensitivity, and application suitability, often involving knockout controls. Researchers also explore BRD8 as a potential therapeutic target, given the growing interest in BET inhibitors for cancer and inflammatory diseases. Its dual bromodomains and unique C-terminal regions distinguish BRD8 from other BET proteins, making selective antibody development critical for precise mechanistic studies.
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