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产品描述：鸡热休克蛋白70(HSP-70)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSP70含量。HSP70是一种重要的热休克蛋白。其背景在于它在细胞应激保护中发挥关键作用，广泛存在于各类细胞。研究机制方面，它能帮助蛋白质正确折叠、防止蛋白聚集，还参与细胞内的多种信号转导途径，在肿瘤、神经退行性疾病等研究中备受关注。试剂盒检测范围为0.25 ng/mL-50 ng/mL，其应用涵盖家禽热应激模型评估、疾病相关蛋白表达调控研究、免疫应答机制探索等科研场景，为禽类生理病理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Heat shock 70 kDa protein ELISA Kit; HSP70 ELISA Kit
缩写：HSP70
Uniprot No.：P08106
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：0.25 ng/mL-50 ng/mL
灵敏度：0.125 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken Hsp-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 83-97 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 84-99 | |||||
回收率：
| The recovery of chicken Hsp-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-104 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 02.5762.5182.547 0.251.4151.3881.402 11.0351.0471.041 40.6100.5980.604 12.50.3290.3140.322 500.1730.1750.174 | ||||||
货期：3-5 working days
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