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产品描述：牛热休克蛋白70(HSP-70)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSP70含量。HSP70是一类广泛存在的热休克蛋白，在机体应对应激、维持细胞内环境稳定等方面有重要作用。研究发现其参与调节蛋白折叠、转运及降解等机制。同时，它与肿瘤发生发展、神经退行性疾病等病理过程密切相关，是潜在的药物靶点。试剂盒检测范围为1.25 ng/mL-20 ng/mL，该产品适用于畜牧科研领域，可用于探究热应激对牛生理机能的影响、疾病模型中细胞应激机制研究，或评估饲养环境因素对牛群健康状态的潜在影响，为动物生理学、病理学及畜牧业管理研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：HSP-70
Uniprot No.：Q27975
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-20 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of bovine HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-89 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.1350.1310.133 100.2190.2310.225 50.4270.4010.414 2.50.7470.7920.770 1.251.7241.6261.675 | ||||||
货期：3-5 working days
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