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产品描述：鱼热休克蛋白70(HSP-70)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSP70含量。HSP70是重要的热休克蛋白，在细胞应激反应中起关键作用，广泛参与蛋白质折叠、转运和降解等过程。研究发现其异常表达与肿瘤、神经退行性疾病等多种疾病相关，抑制或激活HSP70功能正成为潜在治疗策略与研究热点。试剂盒检测范围为18.75 pg/mL-300 pg/mL，该产品为科研人员提供可靠工具，可应用于水产养殖健康监测、环境压力评估、鱼类生理机制研究等领域，为水生生物应激反应模型的建立及生态毒理学实验提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit
缩写：HSP-70
Uniprot No.：Q9I8F9
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-300 pg/mL
灵敏度：18.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 82 | ||||
| Range % | 80-85 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of fish HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 94 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 3000.1530.1440.149 1500.2550.2420.249 750.5190.5330.526 37.51.1031.1341.119 18.752.3632.2702.317 | ||||||
货期：3-5 working days
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