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Human Heat Shock Protein 72(HSP-72) ELISA Kit

  • 中文名： 人热休克蛋白72(HSP-72)ELISA Kit
货号: IPD-E14916h
Price： ￥2500
数量：
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产品详情

  • 产品描述：人热休克蛋白72(HSP-72)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的HSP72含量。HSP72，即热休克蛋白72，是一种在细胞应激反应中上调的分子伴侣，具有广泛的细胞保护功能。它在多种生理和病理过程中发挥作用，如肝脏保护、免疫调节和细胞凋亡抑制。HSP72的研究机制涉及其对细胞内蛋白质的折叠、组装和运输的调节，以及其对抗氧化应激和细胞损伤的保护作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，该产品适用于体外科研场景，例如探究细胞应激反应机制、疾病模型中HSP-72的动态表达规律，或用于药物开发中化合物对HSP通路影响的评估，为分子生物学、病理生理学及药理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：HSPA1A ELISA Kit; HSP72 ELISA Kit; HSPA1 ELISA Kit; HSX70Heat shock 70 kDa protein 1A ELISA Kit; Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit; HSP70.1 ELISA Kit

  • 缩写：HSP-72

  • Uniprot No.：P0DMV8

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, cell culture supernates, cell lysates

  • 检测范围：3.12 ng/mL-200 ng/mL

  • 灵敏度：0.78 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Signal Transduction

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %84
    Range %80-90
    1:2Average %98
    Range %91-103
    1:4Average %101
    Range %92-108
    1:8Average %93
    Range %86-96

  • 回收率：

    The recovery of human HSP-72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9287-98
    EDTA plasma (n=4)9992-107














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected 2002.8682.8562.8622.706 1002.4362.3872.4122.256 501.6941.6681.6811.525 251.0341.0241.0290.873 12.50.5940.5870.5910.435 6.250.4150.4020.4090.253 3.120.2830.2730.2780.122 00.1580.1540.156　 






  • 货期：3-5 working days

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