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产品描述：人热休克蛋白70(HSP-70)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSPA1B含量。HSPA1B 是重要的应激蛋白，在细胞内发挥分子伴侣作用。它在细胞应激反应、蛋白质折叠与转运等方面有重要功能。研究发现其表达水平与多种疾病相关，如肿瘤，深入研究其作用机制，有望为疾病诊断、治疗及药物研发提供新方向。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外科研场景中HSP - 70的动力学研究，例如热休克反应机制探索、药物处理或环境胁迫下的蛋白表达分析，亦可用于动物模型或细胞实验中的生物标志物定量评估。满足实验室对HSP - 70相关基础研究、疾病机制解析及生物治疗开发等多元化需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HSPA1B ELISA Kit; HSP72 ELISA Kit; Heat shock 70 kDa protein 1B ELISA Kit; Heat shock 70 kDa protein 2 ELISA Kit; HSP70-2 ELISA Kit; HSP70.2 ELISA Kit
缩写：HSPA1B
Uniprot No.：P0DMV9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 85-100 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 95-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-94 | |||||
回收率：
| The recovery of human HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-106 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.5882.5212.5552.445501.7451.6761.7111.601251.1331.1121.1231.01312.50.6670.6210.6440.5346.250.4010.3990.4000.2903.120.2380.2330.2360.1261.560.1670.1590.1630.05300.1120.1080.110 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human HSP70 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled HSP70 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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