首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：牛热休克蛋白72(HSP-72)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSPA2含量。HSPA2属热休克蛋白家族，在细胞应激反应等过程起重要作用。其背景上与细胞增殖、凋亡等密切相关。研究机制方面，它可通过调节蛋白折叠、维持蛋白稳态等影响细胞生物学行为，在肿瘤等疾病研究中是潜在靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于畜牧科研中评估热应激对牛群的影响、疾病模型构建或环境压力下分子调控机制研究，亦可为疫苗开发或营养干预实验提供定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HSPA2 ELISA Kit; HSP70-3 ELISA Kit; HSPA3 ELISA Kit; Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit
缩写：HSPA2
Uniprot No.：P34933
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSP-72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-108 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of bovine HSP-72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5322.4322.4822.349101.9741.8741.9241.79151.2711.1711.2211.0882.50.8050.7850.7950.6621.250.4600.4500.4550.3220.6250.3380.3280.3330.2000.3120.2280.2180.2230.09000.1340.1320.133 | ||||||
货期：3-5 working days
×
