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产品描述:人热休克同源物71kDa蛋白(HSPA8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的HSPA8含量。HSPA8是重要的热休克蛋白,广泛存在于多种细胞中,在蛋白质折叠、转运、降解等过程发挥关键作用。研究表明它与肿瘤发生发展、神经系统疾病等密切相关,可通过调节细胞内蛋白稳态、细胞凋亡等机制参与疾病进程。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,适用于体外培养细胞模型、动物实验样本或临床前研究中HSPA8蛋白水平的动态监测,例如探究药物干预、基因修饰或病理条件下HSPA8的表达变化规律,为分子机制研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:2410008N15Rik ELISA Kit; Constitutive heat shock protein 70 ELISA Kit; Epididymis luminal protein 33 ELISA Kit; Epididymis secretory sperm binding protein Li 72p ELISA Kit; Heat shock 70 kDa protein 8 ELISA Kit; Heat shock 70kD protein 10 ELISA Kit; Heat shock 70kD protein 8 ELISA Kit; Heat shock 70kDa protein 8 ELISA Kit; Heat shock cognate 71 kDa protein ELISA Kit; Heat shock cognate protein 54 ELISA Kit; Heat shock cognate protein 71 kDa ELISA Kit; Heat shock protein 8 ELISA Kit; Heat shock protein A8 ELISA Kit; Heat shock protein family A (Hsp70) member 8 ELISA Kit; Heat-shock70-KD protein 10, formerly ELISA Kit; HEL 33 ELISA Kit; HEL S 72p ELISA Kit; HSC54 ELISA Kit; HSC71 ELISA Kit; Hsc73 ELISA Kit; HSP71 ELISA Kit; HSP73 ELISA Kit; HSP7C_HUMAN ELISA Kit; HSPA10 ELISA Kit; HSPA8 ELISA Kit; LAP1 ELISA Kit; Lipopolysaccharide associated protein 1 ELISA Kit; LPS associated protein 1 ELISA Kit; LPS associated protein ELISA Kit; MGC102007 ELISA Kit; MGC106514 ELISA Kit; MGC114311 ELISA Kit; MGC118485 ELISA Kit; MGC131511 ELISA Kit; MGC29929 ELISA Kit; N-myristoyltransferase inhibitor protein 71 ELISA Kit; NIP71 ELISA Kit
缩写:HSPA8
Uniprot No.:P11142
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:31.25 pg/mL-2000 pg/mL
灵敏度:7.8 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human HSPA8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 96 | |||||
Range % | 89-99 | ||||||
1:2 | Average % | 88 | |||||
Range % | 83-92 | ||||||
1:4 | Average % | 91 | |||||
Range % | 84-95 | ||||||
1:8 | Average % | 100 | |||||
Range % | 91-105 |
回收率:
The recovery of human HSPA8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 89 | 82-94 | |||||
EDTA plasma (n=4) | 98 | 87-103 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected 20001.8681.9131.8911.719 10001.5451.5271.5361.364 5001.1661.1241.1450.973 2500.8030.8250.8140.642 1250.5010.5140.5080.336 62.50.3780.3710.3750.203 31.250.2790.2660.2730.101 00.1750.1690.172 |
货期:3-5 working days
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