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产品描述：小鼠丝氨酸蛋白酶HTRA1(HTRA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HTRA1含量。HTRA1是一种丝氨酸蛋白酶。其与多种疾病相关，如年龄相关性黄斑变性等。研究机制上，它可通过调控细胞外基质、生长因子等发挥作用，参与细胞的增殖、凋亡等过程，针对它的研究有助于探索疾病的发病机制和潜在治疗靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，支持科研人员便捷地开展动物模型中的HTRA1动态监测，如中枢神经系统损伤修复研究、肿瘤微环境分析或心血管疾病机制探索，为蛋白表达调控、药物干预效果评估等实验提供可靠数据支持，适用于分子生物学、基础医学等领域的非诊断性研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Htra1 ELISA Kit; Htra ELISA Kit; Prss11 ELISA Kit; Serine protease HTRA1 ELISA Kit; EC 3.4.21.- ELISA Kit; High-temperature requirement A serine peptidase 1 ELISA Kit; Serine protease 11 ELISA Kit
缩写：HTRA1
Uniprot No.：Q9R118
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HTRA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of mouse HTRA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 100-110 | |||||
| EDTA plasma (n=4) | 93 | 89-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected50002.6352.4922.5642.37425002.0011.9031.9521.76212501.3291.3571.3431.1536250.8140.8600.8370.6473120.5470.5190.5330.3431560.3520.3380.3450.155780.2630.2710.2670.07700.1860.1940.190 | |||||||
货期：3-5 working days
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