首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人亨廷顿蛋白(HTT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HTT含量。HTT是亨廷顿病相关重要靶点。亨廷顿病是神经退行性疾病，由HTT 基因变异所致。研究主要围绕调控突变HTT蛋白表达、清除异常聚集等机制，为开发能延缓或阻止病情进展的药物提供了方向。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于细胞/动物疾病模型中HTT蛋白动态监测、基因干预效果评估、病理组织样本检测等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AI256365 ELISA Kit; C430023I11Rik ELISA Kit; HD ELISA Kit; HD protein ELISA Kit; HD_HUMAN ELISA Kit; HDH ELISA Kit; HTT ELISA Kit; Huntingtin ELISA Kit; HUNTINGTON CHOREA ELISA Kit; Huntington disease protein ELISA Kit; Huntington's disease protein homolog ELISA Kit; IT 15 ELISA Kit; IT15 ELISA Kit; OTTMUSP00000026909 ELISA Kit; ZHD ELISA Kit
缩写：HTT
Uniprot No.：P42858
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HTT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:8 | Average % | 110 | |||||
| Range % | 104-114 | ||||||
回收率：
| The recovery of human HTT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-99 | |||||
| EDTA plasma (n=4) | 103 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected8002.2452.1982.2222.1344001.7421.7651.7541.6662001.2781.3011.2901.2021000.8900.8910.8910.803500.6150.6040.6100.522250.4110.4210.4160.32812.50.2830.2860.2850.19700.0890.0860.088 | |||||||
货期：3-5 working days
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