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产品描述：人透明质酸酶-2(HYAL2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HYAL2含量。HYAL2是一种靶点。它是透明质酸酶家族成员，在细胞外基质代谢等过程中有重要作用。研究发现其与肿瘤发生、发展等机制相关，可能参与肿瘤细胞增殖、迁移及免疫逃逸，是肿瘤治疗等领域潜在研究靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，细胞生物学、疾病机制研究、肿瘤微环境调控、炎症性疾病、组织纤维化研究等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hyal 2 ELISA Kit; Hyal-2 ELISA Kit; Hyal2 ELISA Kit; HYAL2_HUMAN ELISA Kit; Hyaluronidase 2 ELISA Kit; Hyaluronidase 2 Precursor ELISA Kit; Hyaluronidase-2 ELISA Kit; Hyaluronoglucosaminidase 2 ELISA Kit; Hyaluronoglucosaminidase-2 ELISA Kit; LUCA 2 ELISA Kit; LUCA-2 ELISA Kit; LUCA2 ELISA Kit; Lung carcinoma protein 2 ELISA Kit; lysosomal hyaluronidase ELISA Kit; PH 20 homolog ELISA Kit; PH-20 homolog ELISA Kit; PH20 homolog ELISA Kit
缩写：HYAL2
Uniprot No.：Q12891
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HYAL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:10 | Average % | 98 | ||||
| Range % | 84-102 | |||||
| 1:20 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human HYAL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 99-108 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.3822.3012.3422.185101.8521.8021.8271.67051.1701.1951.1831.0262.50.7890.8370.8130.6561.250.4710.4580.4650.3080.6250.3350.3450.3400.1830.3120.2270.2130.2200.06300.1600.1540.157 | ||||||
货期：3-5 working days
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