首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人ICOS配体(ICOSLG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ICOSLG含量。ICOSLG，也称为B7-H2或GL50，是一种跨膜糖蛋白，属于B7配体家族。它在多种抗原呈递细胞和非淋巴细胞上广泛表达，作为T细胞共刺激分子，对T细胞的激活、增殖、分化和细胞因子产生起重要作用。研究机制涉及ICOSLG与ICOS受体的相互作用，以及其在免疫调节和疾病发生中的作用。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，该产品适用于免疫调节机制研究、肿瘤免疫治疗探索以及生物标志物分析等科研场景，为探索ICOSLG在免疫系统中的功能及调控网络提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ICOSLG; B7H2; B7RP1; ICOSL; KIAA0653; ICOS ligand; B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD antigen CD275
缩写：ICOSLG
Uniprot No.：O75144
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.4 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ICOSLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 89-102 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 95-106 | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of human ICOSLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 88-103 | ||||
| EDTA plasma (n=4) | 94 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected60002.7482.7892.7692.59230002.3752.3722.3742.19715001.8341.8161.8251.6487501.0611.0471.0540.8773750.6320.6360.6340.457187.50.4270.4410.4340.25793.750.2590.2690.2640.08700.1790.1740.177 | ||||||
货期：3-5 working days
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