| 纯度 | ＞85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TYW5 |
| Uniprot No | A2RUC4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-315aa |
| 氨基酸序列 | MAGQHLPVPR LEGVSREQFM QHLYPQRKPL VLEGIDLGPC TSKWTVDYLS QVGGKKEVKI HVAAVAQMDF ISKNFVYRTL PFDQLVQRAA EEKHKEFFVS EDEKYYLRSL GEDPRKDVAD IRKQFPLLKG DIKFPEFFKE EQFFSSVFRI SSPGLQLWTH YDVMDNLLIQ VTGKKRVVLF SPRDAQYLYL KGTKSEVLNI DNPDLAKYPL FSKARRYECS LEAGDVLFIP ALWFHNVISE EFGVGVNIFW KHLPSECYDK TDTYGNKDPT AASRAAQILD RALKTLAELP EEYRDFYARR MVLHIQDKAY SKNSE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TYW5重组蛋白的示例参考文献(注：以下内容为虚构示例，实际文献需通过学术数据库检索获取)：
1. **"Expression and Functional Characterization of Recombinant TYW5 in Eukaryotic Systems"**
*作者：Smith J, et al.*
*摘要*：本研究报道了TYW5重组蛋白在哺乳动物细胞中的高效表达与纯化，证实其在tRNA羟化修饰中的催化活性，并分析了其酶动力学特性。
2. **"Structural Insights into TYW5's Role in Wybutosine Biosynthesis"**
*作者：Chen L, et al.*
*摘要*：通过X射线晶体学解析了TYW5重组蛋白的三维结构，揭示了其与底物结合的关键位点，为理解其在酵母tRNA修饰中的分子机制提供依据。
3. **"TYW5 Recombinant Protein Restores tRNA Function in Deficiency Models"**
*作者：Wang Y, et al.*
*摘要*：利用重组TYW5蛋白在细胞模型中修复tRNA羟化缺陷，证明其潜在应用价值于相关遗传性疾病的治疗研究。
4. **"Probing the Catalytic Mechanism of TYW5 Using Site-Directed Mutagenesis"**
*作者：Kim H, et al.*
*摘要*：通过定点突变结合体外酶活实验，阐明了TYW5重组蛋白中关键氨基酸残基对其催化功能的影响，提出了可能的反应路径。
建议通过PubMed、Google Scholar等平台以“TYW5 recombinant protein”为关键词检索最新文献。
TYW5 (tRNA wybutosine-synthesizing protein 5) is a conserved eukaryotic enzyme critical for post-transcriptional modification of tRNA. It participates in the biosynthesis of wybutosine, a hypermodified guanosine derivative found at position 37 of eukaryotic tRNAPhe. This modification enhances translational fidelity by stabilizing codon-anticodon interactions during ribosome decoding, particularly preventing frameshifting errors in mRNA translation.
The human TYW5 gene encodes a 326-amino-acid protein belonging to the Rad6 family of ubiquitin-conjugating (E2) enzyme homologs. While retaining a catalytic cysteine residue in its core UBC domain, TYW5 lacks canonical ubiquitination activity. Instead, its C-terminal STAN (SRA_TYW5 N-terminal) domain facilitates interactions with other enzymes in the wybutosine pathway, including TYW1 and TYW3. Structural studies reveal conformational flexibility critical for coordinating multi-step modification processes.
Recombinant TYW5 proteins are typically produced in E. coli or mammalian expression systems for functional studies. Research focuses on its role in neurological disorders, as TYW5 mutations correlate with intellectual disability and microcephaly. Cancer studies also implicate dysregulated tRNA modification in oncogenic translation programs. In vitro reconstitution of wybutosine biosynthesis using recombinant TYW5 has advanced understanding of translation quality control mechanisms. Current applications include screening small molecules targeting tRNA modification pathways and engineering hyperaccurate translation systems for synthetic biology. As a model enzyme, TYW5 continues to illuminate connections between tRNA epigenetics, proteome integrity, and disease.
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