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产品描述：兔子基质金属蛋白酶抑制因子1(TIMP-1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TIMP1含量。TIMP1即金属蛋白酶组织抑制因子1，在细胞外基质代谢、肿瘤生长转移等过程中扮演重要角色。研究机制方面，它可抑制基质金属蛋白酶活性，调节细胞增殖、凋亡等。当前围绕它在疾病诊断、治疗靶点等方向开展诸多研究，有望为相关疾病干预提供新思路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可满足体外实验中对TIMP - 1表达水平的动态监测需求，例如在细胞模型或动物实验中探究炎症反应、组织重塑或疾病进展相关机制，尤其适用于复杂生物样本中TIMP - 1的科研级定量研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
缩写：TIMP1
Uniprot No.：P20614
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, cell culture supernates , tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:200 | Average % | 102 | ||||
| Range % | 98-108 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of rabbit TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.0860.0840.085 5000.1450.1410.143 2500.2450.2380.242 1250.3920.4130.403 62.50.7250.7270.726 31.20.9630.9440.954 15.61.1541.1791.167 01.7341.6621.698 | ||||||
货期：3-5 working days
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