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产品描述：绵羊肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNF含量。TNF（肿瘤坏死因子）是一种多功能促炎细胞因子，最初因能引起肿瘤组织坏死而得名。其超家族包括19个配体和29个受体，参与宿主防御、炎症、细胞凋亡等生理过程。TNF通过与其受体结合，激活下游信号通路，调节细胞存活、凋亡和炎症反应。试剂盒检测范围为3.125 pg/mL-50 pg/mL，TNF-α的动态监测对绵羊感染性疾病模型、疫苗研发及免疫机制研究具有重要价值；可高效支持动物医学研究中炎症相关疾病模型的建立、病原体感染后免疫应答评估以及抗炎药物作用机制的体外实验，为畜牧科研中的免疫调控研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
缩写：TNF
Uniprot No.：P23383
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：3.125 pg/mL-50 pg/mL
灵敏度：3.125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-109 | |||||
回收率：
| The recovery of sheep TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-95 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 500.1610.1650.163 250.3080.3240.316 12.50.5510.5120.532 6.251.0120.9820.997 3.1252.0542.0112.033 | ||||||
货期：3-5 working days
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