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Human Troponin I, fast skeletal muscle(TNNI2) ELISA kit

  • 中文名： 人肌钙蛋白I，骨骼肌快肌(TNNI2)酶联免疫试剂盒
货号: IPD-EL024012HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人肌钙蛋白I，骨骼肌快肌(TNNI2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的TNNI2含量。TNNI2 即心肌肌钙蛋白 I2，它作为心肌收缩调节的关键蛋白，与心肌功能紧密相关。在研究机制上，其异常表达或突变会影响心肌收缩舒张功能，在心肌疾病发生发展中起重要作用，是心血管疾病研究的潜在靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于肌肉发育、运动损伤模型、肌病机制等基础研究领域。科研人员可通过定量分析不同生理或病理状态下骨骼肌损伤标志物TNNI2的动态变化，为探索肌肉再生机制或药物干预效果提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AMCD 2B ELISA Kit; AMCD2B ELISA Kit; DA 2B ELISA Kit; DA2B ELISA Kit; fast skeletal muscle ELISA Kit; Fast twitch skeletal muscle troponin I ELISA Kit; fast-twitch isoform ELISA Kit; FSSV ELISA Kit; fsTnI ELISA Kit; TNNI 2 ELISA Kit; Tnni2 ELISA Kit; TNNI2_HUMAN ELISA Kit; tro ELISA Kit; Troponin I ELISA Kit; Troponin I fast skeletal muscle ELISA Kit; Troponin I fast twitch 2 ELISA Kit; Troponin I fast twitch isoform ELISA Kit; Troponin I fast twitch skeletal muscle isoform ELISA Kit; Troponin I skeletal fast ELISA Kit; Troponin I type 2 (skeletal fast) ELISA Kit; Troponin I type 2 ELISA Kit
  • 缩写：
    TNNI2
  • Uniprot No.：
    P48788
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma,tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TNNI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    85


    Range %

    81-90


    1:2

    Average %

    93


    Range %

    88-99


    1:4

    Average %

    92


    Range %

    88-97


    1:8

    Average %

    91


    Range %

    86-96


  • 回收率：

    The recovery of human TNNI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    87-97


    EDTA plasma (n=4)

    98

    91-104


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    400

    2.236

    2.334

    2.285

    2.127


    200

    1.642

    1.667

    1.655

    1.497


    100

    1.227

    1.288

    1.257

    1.100


    50

    0.857

    0.829

    0.843

    0.686


    25

    0.610

    0.621

    0.616

    0.458


    12.5

    0.429

    0.453

    0.441

    0.283


    6.25

    0.244

    0.234

    0.239

    0.081


    0

    0.160

    0.155

    0.158




  • 货期：
    3-5 working days

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